氨基酸-壳聚糖荧光碳点基因载体的构建及肿瘤诊疗一体化研究

The key issues for cancer therapy is to develop safe yet high efficient diagnostic and therapeutic modalities. In this project, for high efficient gene delivery and bioimaging application, zwitterionic amino acids passivated fluorescent carbon dots based on biocompatible chitosan are to be synthesized. To realize targeted gene transaction, the further experiment was designed to introduce the functional group (folic acid and biotin) onto the surface of fluorescent carbon dots. The combination of tumor-targeted imaging and therapy in an all-in-one system provides a useful approach in the battle against cancer. It is helpful to provide effective information and methods for tumor diagnosis and treatment by studying the transfection mechanism and structure-activity relationships between carbon dots and their fluorescence properties, transfection efficiency and anti-tumor activity.

发展安全高效的诊疗手段是攻克肿瘤的关键。本项目研究拟以生物兼容性的壳聚糖为碳源、两性的氨基酸为钝化剂，制备具有两性离子化特性和高荧光性能的碳量子点基因载体。通过进一步的靶向修饰，构建兼具运载和示踪功能的靶向型基因载体。组合碳点的荧光特性、运载功能以及传递基因的治疗作用，协同实现对肿瘤的诊疗一体化。深入研究该类新型基因载体与荧光性能、转染效率和抗肿瘤活性之间的构效关系及转运机制，为肿瘤诊断和治疗提供有效的信息和手段。

攻克肿瘤的关键在于发展安全高效的诊疗手段。本项目研究以生物兼容性的壳聚糖为碳源、两性的氨基酸为钝化剂，制备具有两性离子化特性和荧光性能的碳量子点基因载体。通过金属掺杂，可进一步改善碳点的光谱行为和荧光效率。组合碳点的荧光特性、运载功能以及传递基因的治疗作用，可协同实现对肿瘤的有效治疗。本项目研究可为基因转运机理研究和肿瘤治疗提供有效的信息和手段。....