基于转录组学的柑橘响应黄龙病菌侵染的功能基因发掘和表达研究

Huanglongbing (HLB, Citrus Greening) is the most destructive disease of citrus in China and worldwide, which causes large damage to citrus industry. Improving the resistance level of citrus to Huanglongbing is a problem in citrus resistance breeding that should be solved urgently. The molecular mechanism of Candidatus?Liberibacter asiaticus(Ca. Las) mediated defence response in citrus remains unknown, that greatly restrict progress in citrus resistance genetics. Molecular biology methods based on transcriptome, Bioinformatics data analysis (De Novo, Unigene alignment, new gene/SNP/alternative splicing identification, annotion, GO category, KEGG pathway analysis of Unigenes or differentially expressed genes), phytopathology technics based on plant-pathogen interaction would be applied in this study to screen functional genes mediated by Ca. Las in susceptible or resistant citrus trees. All the functional gene and protein data obtained would be used for analysing Ca. Las induced citrus defence/tolerance molecular pathways. Selected key regulators would be combined with the renewed pathways and provided to enhance understanding of citrus stress/defense responses to HLB. This study is aiming at offering useful information for citrus HLB medical control and molecular breeding.

柑橘黄龙病是世界及我国柑橘生产中的毁灭性病害，严重影响了柑橘产业的发展。改良柑橘黄龙病抗性是我国柑橘抗病育种中亟待解决的问题，但是目前黄龙病相关病原（Candidatus Liberibacter asiatucus, Ca. Las）在柑橘中的致病关系及柑橘对该病原菌的防御反应的分子研究基础还很薄弱，严重制约了柑橘抗性遗传研究进展。本项目拟综合利用转录组学（转录组测序）、生物信息学（Unigenes的组装、功能注释、Gene Ontology分析、代谢通路分析、编码蛋白框预测、表达差异分析等）和分子植物病理学（病原物的分子检测、病程相关基因的表达分析及克隆）的原理与方法，高通量地筛选抗、感黄龙病的柑橘应答Ca. Las的功能基因；揭示黄龙病相关菌Ca. Las介导的柑橘抗、感病分子机理和关键调控因子及柑橘应答Ca. Las的防御反应途径；将为黄龙病抗病育种提供一定的理论基础。

柑橘黄龙病是世界及我国柑橘生产中的毁灭性病害，严重影响了柑橘产业的发展。改良柑橘黄龙病抗性是我国柑橘抗病育种中亟待解决的问题，但是目前黄龙病相关病原（“Candidatus Liberibacter asiatucus”, “Ca. Las”）在柑橘中的致病关系及柑橘对该病原菌的防御反应的分子研究基础还很薄弱，严重制约了柑橘抗性遗传研究进展。本项目综合利用基因组学（基因芯片）、转录组学（转录组测序）、生物信息学（Unigenes的组装、功能注释、Gene Ontology分析、代谢通路分析、编码蛋白框预测、表达差异分析等）和分子植物病理学（病原物的分子检测、病程相关基因的表达分析及克隆）的原理与方法，高通量地筛选到了一组柑橘严重感染黄龙病后的分子反应途径相关基因，筛选获得一组具有不同黄龙病抗性的柑橘应答“Ca. Las”的功能基因；初步揭示黄龙病相关菌“Ca. Las”侵染柑橘的分子机理和关键调控因子及柑橘应答“Ca. Las”的防御反应途径；为黄龙病抗病育种提供一定的理论基础。