囊泡病毒调控媒介寄生蜂的寄主选择识别机制研究

Nowadays efficient biological control of important vegetable pests such as Spodoptera litura and Spodoptera exigua has become key point for agricultural development. In order to develop safe and new bio-control materials, this project aims at studying the newly discovered Heliothis virescens ascovirus 3h (HvAV-3h). It is still short, the entire process of transmission depended by parasitic wasp has not been completely understood and a few reports have been reported. We base on the theory of insect host selection behavior by insect-borne plant viruses, from the direct indirect impact to carry out research. At the same time, by investigating the effects of virus on host nutrients, host volatiles, parasitic wasp-targeting plants, and host selecting preference, we clarify the ecological and biochemical mechanism of HvAV-3h manipulating the vector's host selection behavior. This project can creat a theoretical foundation for the interaction between insect viruses, vector, host and plant. Then it would provide a scientific basis for the efficient use of HvAV-3h in the control of important vegetable pests such as beet armyworm.

当今，斜纹夜蛾、甜菜夜蛾等重要蔬菜害虫的高效生物防控已成为农业产业发展的关注重点。为开发新型安全的生物防治材料，本项目针对近年来自我国本土新发现的高致病性烟芽夜蛾囊泡病毒3h毒株（Heliothis virescens ascovirus 3h，HvAV-3h），基于其发现时间尚短、依赖寄生蜂传播的整个过程尚未完全清晰、其调节媒介昆虫寄主选择识别方面的报道很少等原因，借鉴虫媒植物病毒调控媒介昆虫寄主选择行为的理论，从昆虫病毒（HvAV-3h）直接影响鳞翅目害虫和间接影响植物两方面开展研究。通过对病毒影响宿主营养、宿主挥发物、寄生蜂定位植物和宿主选择偏向性这四个角度进行分析，明确HvAV-3h调节斯氏侧沟茧蜂寄主选择行为的生态与生化机制。为今后深入研究“昆虫病毒-媒介-宿主-植物”系统互作关系奠定理论基础，更为田间高效利用HvAV-3h防控甜菜夜蛾等重要蔬菜害虫提供科学依据。

当今，斜纹夜蛾、棉铃虫等重要蔬菜害虫的高效生物防控已成为农业产业发展的关注重点。为开发新型安全的生物防治材料，本项目针对近年来自我国本土新发现的高致病性烟芽夜蛾囊泡病毒3h毒株（Heliothis virescens ascovirus 3h，HvAV-3h），基于其发现时间尚短、依赖寄生蜂传播的整个过程尚未完全清晰、其调节媒介昆虫和寄主害虫选择行为方面的报道很少等原因，从昆虫病毒（HvAV-3h）直接影响鳞翅目害虫和间接影响植物两方面开展研究，并通过对病毒影响宿主营养、宿主挥发物、寄生蜂定位植物和宿主选择偏向性这四个角度进行分析，明确了HvAV-3h调节斯氏侧沟茧蜂和斜纹夜蛾寄主选择行为的生态与生化机制。除此之外，明晰了大白菜利用虫害诱导挥发物抵抗斜纹夜蛾危害的化学生态学机制，并发现了同一物种（辣椒）的不同品种具备不同抗虫机制。这些发现为后续分子机制研究奠定了基础，更为田间高效利用HvAV-3h防控重要蔬菜害虫提供科学依据。