GmFHY3/GmFAR1基因在大豆光周期调控开花途径中的作用

Soybean photoperiodic response directly affect the growth period and adaptability, which is closely related to yield. Gene cloning and functional Elucidating about photoperiodic flowering genes (E1, E3 and E4) in soybean revealed that photoperiodic response involving Phytochrome A(PHYA) regulatory pathways. But E3(GmPHYA3) and E4 (GmPHYA2) how to regulate E1 is still not clear. FHY3/FAR1 of transposase-derived transcription factors as positive regulatory in PHYA light signaling of Arabidopsis provided an optimal opportunity to study molecular function of GmFHY3/GmFAR1 in soybean photoperiodism. This project purpose is that characterized status of GmFHY3/GmFAR1 in PHYA signaling pathway (E3, E4 and E1) and investigated which copies participate in E3 or E4 pathway. This research strategies and objectives based on following works: 1), Analysis expressional patterns of GmFHY3/GmFAR1 in different day length, different light treatment (red, far-red, blue and natural light) and different combined alleles of E1, E3 and E4 genotype; 2), Investigate tissue specific expression and subcellular localization of five GmFHY3/GmFAR1 genes; 3), Construct and transform 5 GmFHY3/GmFAR1 overexpress vectors into Arabidopsis wild type(col-0) and mutants(far1, fhy3 and far1/fhy3) plants and then observe the phenotype variation; 4), Analysis about phylogenic relationship, genetic diversity in minicore landraces and evolutionary history of GmFHY3/GmFAR1 gene family in soybean through bioinformatics and comparative genomics methods. Based on this works, we finally aim to understand the status and potential biological functions of GmFHY3/GmFAR1 members in E3 and E4 mediated PHYA signaling photoperiodic pathway in soybean. The results will provide great theoretical and practical significance for further elucidate soybean photoperiodic response and breeding.

大豆光周期反应直接影响到生育期及适应性，与产量密切相关。大豆生育期基因(E3，E4与E1)的功能涉及光敏色素A调控通路。但E3(GmPHYA3)与E4(GmPHYA2)如何调控E1仍不明确。本项目拟利用转座子起源的FHY3/FAR1基因在PHYA通路中正向作用为突破口，首先分析不同E1、E3与E4基因型背景下,不同光周期、不同光质处理后5个GmFHY3/GmFAR1家族成员的表达模式，基因组织特异性表达及亚细胞水平定位情况；其次分析这些基因在拟南芥野生型与突变株中过表达的情况；最后对大豆GmFHY3/GmFAR1基因家族进行系统发生、起源方式及在大豆微核心种质中的分布、遗传多样性进行研究。以期明确GmFHY3/GmFAR1基因家族成员在光敏色素A所涉及的光周期反应中的作用及潜在的生物学功能。对深入了解大豆光周期反应机理及开展相应的光周期适应性育种具有重大的理论意义与实际价值。