高迁移率族蛋白(HMG2L1,SOX2)介导FSH促卵巢癌细胞生长与转移作用及其分子机制研究

As we all known,FSH has the positive effects on the proliferation and metastasis of the ovarian cancer cells. Our previouse study has showed that FSH could enhance the exprssions of the important protein molecules which can maintain the low differentiation ，such as OCT4、SOX2and HMG2L1. those results of the study also has showed that OCT4 siRNA could decrease the abilities of the proliferatin and metastasis of the ovarian cancer cells.Transfection 、siRNA、real time PCR、Western Blot and quantify ChIP are used in the study to clarify the mechanism of the effects of SOX2 and HMG2L1 on the proliferatin and metastasis of the ovarian cancer cells. We hope that this study can improve our understanding the hypothesis of the FSH pathogenesis for ovarian cancer and find a successful molecule target for the treatment of ovarian cancer.

目前已知，FSH对卵巢癌细胞生长和转移均具正调节作用。本课题组前期研究发现：FSH也可增加能维持卵巢癌细胞低分化状态的重要蛋白分子的表达，例如OCT4、SOX2和HMG2L1；OCT4 siRNA可降低卵巢癌细胞增生和迁移活性。为阐明SOX2和HMG2L1(高迁移率族蛋白)在FSH正调节卵巢癌细胞生长及转移中的作用，本项目拟采用转染、siRNA、实时PCR、Western Blot、ChIP等方法，探讨SOX2和HMG2L1介导FSH正调节卵巢癌细胞生长及转移的作用及其分子机制，为完善上皮性卵巢癌FSH致病学说的研究提供新的实验依据。同时也可为临床靶向治疗寻找新的、有效靶点。

目前已知，FSH对卵巢癌细胞生长和转移均具正调节作用。本课题组前期研究发现：FSH也可增加能维持卵巢癌细胞低分化状态的重要蛋白分子的表达，例如OCT4、SOX2和HMG2L1；OCT4 siRNA可降低卵巢癌细胞增生和迁移活性。为阐明SOX2和HMG2L1(高迁移率族蛋白)在FSH正调节卵巢癌细胞生长及转移中的作用，本项目采用转染、siRNA、实时PCR、Western Blot、ChIP等方法，探讨SOX2和HMG2L1介导FSH正调节卵巢癌细胞生长及转移的作用及其分子机制，为完善上皮性卵巢癌FSH致病学说的研究提供新的实验依据。同时也可为临床靶向治疗寻找新的、有效靶点。