水稻RCA基因表达与光合作用的关系

RCA expression played an important role in the regulation of Rubisco activity and photosynthesis during rice leaf development and senescence, and high expression of RCA mRNA is relative to rice resistant to premature. There are different mechanisms of RCA expression at the levels of transcription and translation. The fammer is reglated by both light-darkness alternation and an oscillating circadian rhythm while latter is only controlled by light. The results showed that Rubisco mainly located in chloroplast, and RCA were found both in chloroplast and mitochondria. The density of RCA in chloroplast reached the maximum at 14:00 and a valley at 11:00. The result was more coincided with the activity of RCA in leaf. In mitochondria, the density of RCA changed abruptly in one day with the highest at 13:00 and it can well elucidate why the activities of Rubisco strangely declined at noon when its amount was increasing. Therefore the cellular localization and/or distribution of Rubisco and RCA during a day is more important for Pn, Rubisco and RCA activity. cDNA array with more than 5000 genes cloned from rice showed a large number of genes including cellgenesis, transloction and photosynthesis as well as more than 700 candidate genes are regulated by light-darkness alternative. Our results indicated about 3% genes are circadian rhythm genes

利用已制备的水稻核酮糖二磷酸羧化酶/加氧酶活化酶(RCA)蛋白抗体和克隆的cDNA作为探针，研究该酶在不同光合速率水稻品种发育时期的时空表达和活性关系，部分外界因素对表达的影响，遗传多态笥分析和转基因研究。进一步明确RCA在提高光合速率及抗病方面的作用Ｕ舛匀娌鞲妹傅墓δ芎捅泶锏鹘诩疤剿魈岣吖夂纤俾实耐揪队兄匾庖濉