青藤碱抑制C5a诱导肾小球系膜细胞促炎因子生成的机制研究

The mesangial proliferative glomerulonephritis（MsPGN）is an inflammatory disease of the glomeruli associated with the immune system, and is serious harmful to health，while PKC/Keap1/Nrf2 signaling pathway participating in the MsPGN inflammation. PKC/Keap1/Nrf2 signaling pathway, leading to low-expression of NF-κB, inhibiting the release of inflammatory cytokines and kidney inflammation, plays an important role in the occurrence and development of MsPGN. Early studies showed that: sinomenine can relieve glomerular inflammation, with higher efficacy and lower side effects, and no liver damage was found. The mechanism is associated with the regulation of NF-κB expression in kidney, whether with PKC/Keap1/Nrf2 pathway remains to be elucidated. In this study, both in vitro and in vivo, we observe PKC/Keap1/Nrf2 mediated signaling pathway to inhibit the expression of NF-κB, to relieve kidney inflammation. We clarify the immunological mechanisms of cure methods and in-depth study to MsPGN in traditional Chinese medicine treatment with sinomenine, in the same time to further improve MsPGN efficacy of treatment, to provide ideas in treatment options for MsPGN.

系膜增生性肾小球肾炎（MsPGN）是一种严重危害人类健康且与免疫相关的肾小球炎性疾病， 而PKC/Keap1/Nrf2信号通路参与了肾脏炎症的发生发展。PKC/Keap1/Nrf2信号通路激活后抑制NF-κB信号活化，抑制下游促炎因子的表达，从而抑制肾脏炎症，在系膜增生性肾小球肾炎的炎症防治中起着重要作用。前期研究表明：青藤碱具有减轻肾小球炎症的作用，具有较高疗效，且副作用小，没有肝损害，其作用机制与下调肾组织NF-κB表达有关，但与PKC/Keap1/Nrf2通路的关系有待进一步阐明。本研究采用从体内外两个方面展开研究，观察青藤碱通过PKC/Keap1/Nrf2通路抑制NF-κB信号从而减轻肾脏炎症反应，阐明青藤碱治疗系膜增生性肾小球肾炎的免疫学机制，为治疗中药的深入研究提供科学依据，为完善系膜增生性肾小球肾炎的现有治疗方案提供思路。