寻常型天疱疮免疫遗传机制研究

Firstly, we analyzed HLA DR, DRB1 and DQB1 polymorphism in patients with pemphigus vulgaris(PV) and found HLA DR4, DR14 antigen and DRB1*0402, DRB1*1401 subtypes are related to Chinese PV susceptibility. Secondly, we investigated Dsg3 antigen determinants in antigen-antibody and cellular response. Recombinant Dsg3 antigen were expressed and identified by westernblot, then we tested the antigen-antibody response using Dsg3 peptides and PV patients sera, isolated T lymphocytes from PV patients and studied the proliferation to Dsg3 antigen. The results showed that there were antigen-antibody response epitopes in Dsg3 E1,E2 and E4, T-B interaction related epitopes existed in Dsg3 E1, E4 and E5. These epitopes interact with HLA DRB1*0402 or DRB1*1401 to present disease related antigen, playing an important role in PV pathogenesis.

寻常型天疱疮（PV）是一种发生于皮肤、粘膜的自身免疫性大疱性疾病，患者中常出现抗跨膜桥粒核心糖蛋白3（Dsg3）抗体，其发病机理不清，涉及遗传学因素和免疫反应机制。我悄庋芯课夜鶳V易感性HLA基因，分析PV患者抗体反应和特异性T细胞反应与HLA等位基因之间的关系，为PV的治疗提供分子水平上的理论依据。