基于放大技术及免标记的肿瘤标志物及肿瘤细胞单分子检测方法研究

In the present work, label free detection strategies will be constructed for the determination of tumor cells and tumor markers based on the Quartz Crystal Microbalance (QCM).The chips of QCM will be modified with biotin or other bionics compound to enhance the amount of capture probes anchored on the surface of the chips. Rolling Circle Ampli?cation (RCA) technology and nanoparticles will be used to realize the single molecule detection of the tumor markers and cancer cells. Multiple target detection will be studied by using the multiple biosensors.The proposed label free ampli?cation strategy would become a powerful tool for proteomics research and early clinical diagnostics.

本项目拟基于免标记的石英晶微天平（QCM）技术，利用生物素及各种生物膜对芯片进行仿生修饰，研究捕获分子在芯片上的结合能力，提高捕获分子的固载量；结合滚环复制放大技术及纳米粒子放大技术，构建新型的免标记生物传感器，提高检测的灵敏度和选择性，实现复杂体系中肿瘤标志物及肿瘤细胞的单分子检测，并将通过构建多种识别探针，实现多靶标同时检测，为恶性肿瘤的早期诊断和早期治疗提供理论基础。

本项目基于酶循环放大技术及纳米粒子放大技术，构建新型的免标记生物传感器。修饰有适体等化合物的纳米颗粒与磁珠上的捕获探针相结合，再利用循环放大技术，提高检测的灵敏度和选择性，实现复杂体系中肿瘤标志物及肿瘤细胞内肿瘤标志物的检测，并将通过构建多种识别探针，实现多靶标同时检测，为恶性肿瘤的早期诊断和早期治疗提供理论基础。