VEGF在人脐带间充质干细胞修复受损子宫内膜中的作用及作用机制

Intrauterine adhesions, amenorrhea, and infertility caused by endometrial repair obstacles has become an important issue. It has an important clinical significance in improving the environment of the uterine cavity, facilitating restoration of menstruation and increasing pregnancy rates. Animal experiments have shown that mesenchymal stem cells can ameliorate multiple organ damage.However,the systemic research is rare in reparing the damaged endometrium. Our preliminary study found that mesenchymal stem cells derived from the human umbilical cord, Wharton's jelly mesenchymal stem cells (WJ-MSCs) could ameliorate damaged human endometrium induced mifepristone in vivo and in vitro. This project aims to further explore and clarify the repair capability of WJ-MSCs for damaged endometrium and its molecular mechanism. The intracellular and extracellular signal molecules mediated by VEGF in the repairing process of damaged endometrium will be tested in vitro and in vivo. The signal pathway of WJ-MSCs which mediate the endometrium repairing will be detected by the method of signal blocking,gene knockdown,marked by green fluorescent protein(GFP) and transplanted into to rats to further confirm the signal path and the mechanisms by VEGF in ameliorating the damaged endometrium. The distribution and the transdifferentiation of WJ-MSCs will be also observed. The promising results may provide a new direction and therapeutic targets for the restoration of women's endometrium after injury.

子宫内膜损伤后修复障碍已成为女性宫腔粘连、闭经、不孕的重要原因。深入研究其损伤后的结构与功能修复的规律，探索可能的干预措施，对改善子宫内环境、促进月经恢复及提高妊娠率等具有重要的临床意义。动物实验表明，间充质干细胞可以修复多种器官的损伤，但对修复子宫内膜损伤缺少系统的研究。我们的研究发现人脐带华通氏胶间充质干细胞（WJ-MSCs）对体内外米非司酮诱导的子宫内膜损伤模型均具有一定的修复作用。本研究旨在探明WJ-MSCs对子宫内膜的修复作用及其分子机制。在体内、外检测确定参与子宫内膜修复过程的细胞因子VEGF及其调控信号分子；利用信号阻断、基因敲除及将绿色荧光蛋白（GFP）转染标记的WJ-MSCs移植到子宫内膜受损的大鼠体内，并追踪标记细胞的转归等方法检测VEGF介导修复的信号通路及修复机制。通过本课题的研究可以为女性子宫内膜损伤后的结构与功能修复提供一个新的指导方向和治疗。

子宫内膜损伤后修复障碍已成为女性宫腔粘连、闭经、不孕的重要原因。深入研究其损伤后的结构与功能修复的规律，探索可能的干预措施，对改善子宫内环境、促进月经恢复及提高妊娠率等具有重要的临床意义。动物实验表明，间充质干细胞可以修复多种器官的损伤，但对修复子宫内膜损伤缺少系统的研究。我们的研究发现人脐带华通氏胶间充质干细胞（WJ-MSCs）对体内外米非司酮诱导的子宫内膜损伤模型均具有一定的修复作用。本研究旨在探明WJ-MSCs对子宫内膜的修复作用及其分子机制。在体内、外检测确定参与子宫内膜修复过程的细胞因子VEGF及其调控信号分子；利用信号阻断、基因敲除及将绿色荧光蛋（GFP）转染标记的WJ-MSCs移植到子宫内膜受损的大鼠体内，并追踪标记细胞的转归等方法检测VEG介导修复的信号通路及修复机制。通过本课题的研究可以为女性子宫内膜损伤后的结构与功能修复提供一个新的指导方向和治疗。