基于亲和膜吸附-洗脱过程的胆红素检测方法的研究

Serum bilirubin are usually measured to monitor and to judge liver and gall bladder disfunctions. Because of complicated components in the blood system, the conventional determination method of bilirubin owes high disturbance, large test error, and complicated steps, etc. Thus the advancement of bilirubin determination method attracts the attention of researchers. Based on the preparation of affinity membrane and bilirubin adsorption results, caffeine reagent was selected and combined with an alkaline solution to desorb bilirubin from the membrane. Combination of adsorption and desorption process, a conception of a test paper membrane was established. In details, with different initial concentration of bilirubin a paper test membrane changes from light yellow to orange after adsorption. By using the relationship, the initial concentration of bilirubin was estimated initially. Furthermore, it is found that using desorption consequences of bilirubin can exactly determine bilirubin initial concentration. In total, a standardized procedure will be established to determine bilirubin by utilizing a paper test membrane. Based on the dynamic adsorption and desorption data, mass transfer of the combination process was studied and mass transfer kinetics was established, in which the continuity of adsorption and desorption was considered more about. It affords the theory basis to the regeneration and recycles use of the paper test membrane.

血清胆红素水平是诊断肝胆等疾病的重要指标，但是血液成分复杂，胆红素检测受干扰较大，测试误差较大，并且步骤繁琐，因此胆红素测试方法的改进一直备受研究者关注。本项目依据前期亲和膜的制备和胆红素吸附的研究，提出了利用拆分剂咖啡因复配碱液洗脱吸附了血清胆红素的膜，联合亲和膜的吸附-洗脱过程，提出了胆红素试纸膜的概念，首先通过吸附后试纸膜的颜色变化，初步判断血清胆红素的浓度，进一步通过胆红素的洗脱过程，获得准确的血清胆红素浓度，建立胆红素试纸膜测试的标准化流程。基于动态吸附和洗脱数据，深入研究胆红素在吸附和洗脱过程的传质动力学，建立胆红素在亲和膜上吸附-洗脱的传质模型，充分考虑了胆红素吸附-洗脱步骤的连续性，对检测试纸膜的再生和重复利用提供了理论依据。

血清胆红素水平是诊断肝胆等疾病的重要指标，但是血液成分复杂，胆红素检测受干扰较大，测试误差较大，并且步骤繁琐，因此胆红素测试方法的改进一直备受研究者关注。本项目在前期亲和膜吸附胆红素的研究上，提出了基于亲和膜吸附-洗脱过程的胆红素检测方法，具体完成如下工作：提出了咖啡因复配低浓度碱液洗脱血清胆红素的方法，确定了咖啡因试剂的拆分洗脱机理及碱液加速洗脱的作用，复配洗脱液适用于血清等复杂系统胆红素的洗脱，而且应用扩展到多种胆红素吸附膜。制备了紫外辐射接枝膜、染料改性膜、有机无机共混膜，揭示了亲和膜的配基、配基密度及可及性也就是膜的微观结构在胆红素吸附方面的相互作用关系。当微观结构中膜孔径足够大时，增加配基的疏水性和胺基利于胆红素的吸附和洗脱；但是当膜的孔隙率减小到某一个程度，即使长碳链配基也无法达到内部时，膜的可及配基密度决定胆红素的吸附量。建立了血清胆红素吸附膜的初步颜色判断方法，并通过RGB值之和与胆红素溶液浓度建立了函数关系，制备了标准胆红素试纸卡。进一步通过咖啡因复配碱液洗脱胆红素，得到胆红素洗脱量与血清胆红素初始浓度的正比关系，并初步验证其准确性，建立胆红素试纸膜测试的标准化流程。基于动态吸附数据，建立了胆红素在亲和膜上吸附的传质模型，并通过实验数据拟合进行验证。基金标注资助7篇文章，申请了2篇专利，4人次参加国内外会议，并做了2次口头报告，基金实施过程中签订了1项横向项目，进款4.6万。