β-环糊精诱导重烷基苯磺酸盐激发光谱法定量研究

Accurate quantitative analysis of heavy alkylbenzene sulfonates (HABS) is of great significance to the evaluation technique for ASP flooding test and the analysis of environmental aquatic quality. Now, Difficulty in detection and quantification of HABS with strong interferences at extremely low concentrations is gradually becoming the bottleneck for further development. The absorption spectra of HABS indicates that the presence ofβ-cyclodextrin(β-CD) can result in the enhancement of absorption intensities of HABS. A good linearity will be very likely to be obtained between the absorption intensity of the system and the concentration of HABS. Based on this, a novel enlarged absorption spectrometry method for the quick quantitative determination of HASB will be established. The interaction between β-CD and HABS will be investigated by UV, FT-IR, 1H-NMR, etc. which will clarify the mechanism of enhancement of absorption intensities of HABS and the anti-interference ability ofβ-CD. Then, the critical value of the quantitative determination, the precision and the determination ranges of the method will be studied, and it is likely to be that the precision and the determination ranges of this quantitative determination will be greatly improved. This quantification technique will be significant to the quantitative determination of HABS with strong interferences at extremely low concentrations, which will be also significant to the monitoring of ASP flooding test and the analysis of environmental aquatic quality.

准确定量重烷基苯磺酸盐表面活性剂是评价三元复合驱油效果、分析重烷基苯磺酸盐对环境水水质影响的有效途径，但是低浓度、存在干扰作用时的重烷基苯磺酸盐含量难以被准确检测。本项目拟采用β-环糊精诱导重烷基苯磺酸盐，激发其产生显著增强的光谱信号，通过增强的光谱信号与重烷基苯磺酸盐含量的对应关系进行定量研究。借助紫外光谱、红外光谱、核磁共振等表征，探索β-环糊精与重烷基苯磺酸盐分子间的相互作用关系，阐明β-环糊精对重烷基苯磺酸盐的激发机制，进而在理论指导下进行激发光谱定量的精度、范围、临界值、抗干扰能力及干扰作用修正研究，以期显著提高重烷基苯磺酸盐定量的精度和抗干扰能力。为准确定量低浓度、存在干扰作用时的重烷基苯磺酸盐提供理论和方法依据，也为监控三元复合驱和环境水水质提供理论和技术基础。

三采采出水中重烷基苯磺酸盐（HABS）的检测一直面临困难。本课题利用“β-环糊精激发光谱法”解决了低浓度、存在干扰作用时HABS的检测难题，与当前检测HABS的各类方法相比，本方法可大幅度简化操作、抗干扰能力强、检测准确度及检测精度显著提高，相关研究尚未见有系统报道。. 本课题创造性地发现或揭示了：（1）β-环糊精具有诱导HABS产生激发的紫外、荧光或同步荧光光谱信号的特性，采用该激发的光谱信号，HABS的检测误差可降低80%以上，检出限可由10-2 ~ 10-3 mg/L降低至10-4 mg/L；（2）β-环糊精激发光谱法同时具有显著降低HABS的各种定量干扰作用，β-CD激发光谱法可有效降低各类无机盐离子、两性表面活性剂复配体系、聚合物及非离子表面活性剂等干扰物对HABS定量产生的干扰，该方法适用于准确检测三采采出水中（主要是低浓度时）HABS的含量；（3）系统阐释了β-环糊精与HABS之间的作用机制，证实了HABS分子可以沿着β-环糊精的内腔径向大、小口径端2个方向进入β-环糊精的内腔并自发地形成了稳定的包结物，首次明确了β-环糊精内腔的“诱导”效应和“隔离”作用是β-环糊精具有产生激发光谱及降低干扰作用的本因；（4）探讨了β-环糊精包结并激发HABS的可能机理；（5）发现各类干扰物特别是其他类型表面活性剂与HABS之间的作用会显著影响到HABS的临界胶束浓度（cmc），证实了HABS胶束结构的形成及cmc的不确定性是影响检测“强干扰、高浓度（浓度大于cmc）的HABS”的主要干扰因素。. 上述研究结果验证了我们提出的“β-环糊精激发光谱法能准确定量低浓度、存在干扰作用时的HABS”假说，为检测三采采出水中较低浓度（浓度小于cmc）的HABS提供了理论依据和指导方法。