Rhizophagus irregularis对马铃薯疮痂病防控机制的研究

The potato (Solanum tuberosum L.) is the fourth largest food crops in the world. Potato common scab(CS) caused by phytopathogenic Stretomyces species is one of the most important diseases affecting yield and quality of potatoes. This study propose using arbuscular mycorrhizal fungi(AMF) Rhizophagus irregularis to control CS under potted plant conditions. The control effect and disease resistance mechanism of R. irregularis against CS will be determined by tissue anatomy, high-throughput sequencing, q-PCR, HPLC and enzyme activity assay method, etc. Aim to reveal the main ways of AMF for controlling CS, the effects of inoculation with AM fungi on the structure of potato tissue, phenolic acid content in potato tuber epidermis, virulence gene expression of S. scabies, growth and decline of S. scabies flora and quorum-sensing(QS) signal factors in geocaulosphere soil, and the influence on induced systemic resistance-related genes and enzyme activity of potato plant were analyzed. The result will lay the foundations for further study on the molecular mechanism of AMF in controlling CS. In view of the fact that there is no report on the use of AM fungi to control CS at home and abroad, this study will provide a scientific basis for the large-area application mycorrhizal microbial fertilizer for controlling potato common scab. It is of important practical significance.

马铃薯（Solanum tuberosum L.）是全球第四大粮食作物。由植物病原链霉菌引起的马铃薯疮痂病（Common Scab，CS）是影响马铃薯产量和品质的重要病害之一。本研究拟在盆栽条件下利用AM真菌根内球囊霉（Rhizophagus irregularis）对CS进行生物防治。利用组织解剖学、高通量测序、q-PCR、HPLC、酶活测定等方法研究R. irregularis对CS的防治效果和抗病机理。通过分析接种AM真菌对马铃薯组织结构的影响、对表皮酚酸含量的影响、对Streptomyces scabies毒力基因表达的影响、对薯表土中S. scabies菌群消长和群体感应信号因子的影响、对马铃薯植株系统抗性相关基因及酶活的影响，揭示AM真菌防治马铃薯疮痂病的主要途径。为深入研究AM真菌防治马铃薯疮痂病的分子机理奠定基础，鉴于目前国内外还没有利用AM真菌防治CS的报道，本研究能

马铃薯（Solanum tuberosum L.）是全球第四大粮食作物。由植物病原链霉菌引起的马铃薯疮痂病（Common Scab，CS）是影响马铃薯产量和品质的重要病害之一。本项目旨在探究在盆栽条件下接种AM真菌R. irregularis对马铃薯疮痂病的防效和抗病机制。通过接种AM真菌对马铃薯组织结构解剖学的影响、对表皮酚酸含量的影响，对薯表土中S. scabies菌群消长和群体效应信号因子的影响。结果如下：.（1）接种AM真菌R. irregularis降低马铃薯疮痂病的20%左右的发病率，.（2）接种AM真菌R. irregularis能够增加马铃薯周皮层数和韧皮宽度；马铃薯植株的净光合速率；提高马铃薯根际土壤的速效磷含量。.（3）接种AM真菌R. irregularis的马铃薯菌群结构与未接种处理组有着显著差异，并产生大量的几丁质信号物质。.（4）接种AM真菌R. irregularis的马铃薯根际土壤酚酸含量高于未接种AM真菌的处理组一倍的量，AM真菌系统诱导抗性的信号通路与水杨酸途经有关。.