静电纺丝多元杂化纳米膜仿生识别有机锡电化学传感器构建及应用研究

It is important to establish rapid detection technology and study migration and residual behavior for organotin in agro-products. For the advantages of molecularly imprinted polymer and graphene nanomaterials, the electrostatic spinning and molecularly imprinted polymer(MIP) technique will be adopted to screen the best preparation conditions and electrospinning parameters. The MIP nanofiber of organotin will be synthesized, and the mechanism of efficient recognition of organnotin will be studied. Graphene, gold nanoparticles and MIP will be used layer by layer to modify screen printing electrode array, which could be studied by cyclic voltammograms (CV), differential pulse voltammetric (DPV), electrochemical impedance (EIS) techniques for the optimization of electrochemical parameters. Finally, electrochemical detection technology based on molecular imprinting for organotin in agro-products would be developed. the multi-residue sample preparation technology would be delevoped with the utilization of MIP nano membrane to enrich, separate and purify organotins in the agro-products. The source and existing forms of organotin in agro-products will be investigated by the technologies mentioned above.Research on digestion dynamic experiment and residual behavior of organotin pesticides in vegetables will be carried out to clarify the migration and transformation mechanism in agro-products, which will provide technical support and theoretical basis for monitoring organotin pollution in agroproducts.

针对有机锡污染现状，建立有机锡快速检测技术，研究农产品中有机锡迁移转化与残留行为具有重要科学意义。本项目拟利用静电纺丝与分子印迹（MIP）技术，结合印迹类特异性识别及石墨烯等纳米材料的优点，筛选和优化其制备条件及电纺参数，合成对有机锡具有类特异性吸附的MIP纳米膜，研究纳米膜对有机锡的高效识别机理；以石墨烯、金纳米及MIP层层自组装制备有机锡复合膜印刷阵列电极，利用CV、DPV、EIS法研究各层修饰材料对探针灵敏度和响应速率的影响，优选电化学参数，建立适用于农产品中有机锡的分子印迹电化学传感器检测技术；利用MIP纳米复合膜对农产品中有机锡进行富集、分离和净化，从而建立有机锡多残留前处理技术，研究农产品中有机锡的来源及存在形态，开展有机锡农药在蔬菜中消解动态试验和残留行为研究，阐明农产品中有机锡的迁移与转化机制，为农产品中有机锡污染监控提供技术支撑和理论依据。

本项目基于静电纺丝与分子印迹（MIP）技术，结合印迹类特异性识别及石墨烯等纳米材料的优点，建立了农产品中有机锡的分子印迹电化学传感检测技术。首次建立了食品中三丁基氯化锡、三苯基氯化锡等四种物质的LC-MS/MS检测方法，解决了当前测定样品中有机锡需要经过繁琐的衍生化处理问题。合成有机锡化合物MIP纳米复合材料，建立了6种有机锡MISPE-LC-MS/MS检测方法以及基于离子液体/石墨烯/纳米金复合材料多元杂化修饰的有机锡分子印迹传感器，研究了有机锡化合物的电化学特性。通过电化学聚合法制备三环锡分子印迹纳米膜，以电化学还原氧化石墨烯与纳米金功能化的丝网印刷电极为传感平台，构建了高灵敏便携可抛的三环锡丝网印刷电化学传感器，并实现了梨样品中三环锡的快速检测。以电化学还原氧化石墨烯与纳米金功能化的丝网印刷电极传感平台为接收器，以分子印迹聚合物为特异性识别原件，在电极表面电纺形成分子印迹纳米纤维膜，构建的传感器可实现梨样品中的三环锡的快速高灵敏检测。综上所述，本研究为农产品中有机锡的来源及存在形态研究，开展有机锡农药在蔬菜中消解动态试验和残留行为研究，阐明农产品中有机锡的迁移与转化机制，为农产品中有机锡污染监控提供技术支撑和理论依据。