长链AsCsMTP6和CsMTP6转运体调节黄瓜对锰积累及脱毒机制的研究

The high concentration of manganese ion in the soil of facility cultivation seriously restrain the development of cucumber industry in China. Researches about the genes related to manganese detoxification play important roles in the high-efficient production of cucumber. However, researches about molecular mechanism of cucumber manganese detoxification were remain elusive. By biochemistry method in our previous researches, we had identified a heavy metal transporter (CsMTP6) and a lncRNA (AsCsMTP6) from cucumber inbred line‘9930’，which is specifically to manganese transport. In this project, we plan to carry out the analysis of genetic transformation to identify the manganese accumulation and detoxication of AsCsMTP6 and CsMTP6. In addition, we will systematically analyse the relationship between of AsCsMTP6、CsMTP6， gene expression、DNA methylation、histone methylation and manganese accumulation/detoxication. This results would be helpful for illuminating the function of AsCsMTP6-CsMTP6 in manganese accumulation/detoxication of cucumber, and also be useful for uncovering the molecular mechanism of DNA methylayion in regulating AsCsMTP6 and CsMTP6 expression.

设施栽培土壤中锰离子浓度超标严重影响我国黄瓜产业的发展。锰解毒相关基因及调控机制的研究对黄瓜高效生产具有重要的理论和实践意义。黄瓜锰解毒相关分子机制研究较少，申请者前期在黄瓜‘9930’自交系中筛选得到一个对锰高度专一的金属转运体CsMTP6，并且在CsMTP6反义链上发现了一条长链非编码RNA(AsCsMTP6)，但它们具体的分子调控机制未知。本项目拟通过黄瓜遗传转化鉴定AsCsMTP6和CsMTP6参与调节黄瓜植株对锰的积累与脱毒效应，研究两者在黄瓜不同组织中对锰转运与分配的模式，鉴定CsMTP6调节黄瓜植株及果实中锰的富集行为。并且项目将系统研究AsCsMTP6、CsMTP6和DNA甲基化/组蛋白甲基化、基因表达及锰积累之间的关系。项目最终将阐明asCsMTP6和CsMTP6参与黄瓜对锰吸收、积累和脱毒作用，以及DNA甲基化参与调控CsMTP6和AsCsMTP6功能的分子机理。

设施栽培酸性土壤中锰离子浓度超标严重影响我国黄瓜产业的发展。锰解毒相关基因及调控机制的研究对黄瓜高效生产具有重要的理论和实践意义。黄瓜锰解毒相关分子机制研究较少，项目组在黄瓜中筛选得到一个对锰高度专一的金属转运体CsMTP6，并且在CsMTP6反义链上发现了一条长链非编码RNA(AsCsMTP6)，但它们如何精确调控黄瓜对锰离子的转运和解毒机制则未知。本项目通过黄瓜遗传转化、生物化学和分子生物学等技术手段鉴定到AsCsMTP6负调控CsMTP6参与调节黄瓜植株对锰的积累与脱毒效应，项目系统研究了AsCsMTP6在DNA甲基化水平调控CsMTP6表达进而影响黄瓜锰离子积累的机制。项目阐明了AsCsMTP6和CsMTP6参与黄瓜对锰吸收、积累和脱毒作用，以及DNA甲基化参与调控CsMTP6和AsCsMTP6功能的分子机理。