玉米ZmMAPKKK19调控干旱抗性的机理研究

Maize is one of the most important food, economic and fodder crops worldwide. However, its growth and yield are severely hampered by drought stress. In addition to conventional breeding, transgene technology is an effective way to enhance the drought stress tolerance of maize. MAPK signaling cascades are vital signaling pathways in eukaryotes, which are involved in diverse biological processes, such as responses to environmental stresses. Nevertheless, the drought stress tolerance associated MAPK signaling pathway in maize, has not been elucidated. Via transcriptome analysis and subsequent functional characterization, we found that ZmMAPKKK19 was obviously induced by drought and closely correlated with maize drought stress tolerance. By using transgenic technology, forward and reverse genetics, diverse physiological and biochemical methods, the objectives of this research are to identify the complete MAPK signaling cascade mediated by ZmMAPKKK19 in maize, demonstrate the detailed molecular mechanisms of ZmMAPKKK19 in regulating maize drought stress tolerance and create novel drought tolerant inbred lines. This project will provide effective gene resources, theoretical support and germplasm materials for drought-tolerant and high-yield maize breeding.

玉米是重要的粮食、经济及饲用作物，但其生长与产量均受干旱胁迫严重制约。除常规育种外，转基因技术是提高玉米干旱抗性的有效方式。MAPK级联途径是真核生物体内一种重要的信号转导方式，参与环境胁迫应答等众多生物学过程。但玉米中与干旱抗性密切相关的MAPK途径仍未阐明。通过转录组分析，我们分离鉴定了玉米中受干旱胁迫明显诱导的基因ZmMAPKKK19，并证实该基因与玉米干旱抗性密切相关。本项目以ZmMAPKKK19为研究对象，通过转基因技术、正向与反向遗传学、生理生化指标检测等方法，阐释玉米ZmMAPKKK19介导的完整MAPK信号转导途径及其调控干旱抗性的分子机制，创制抗旱性明显增强的玉米自交系，为实现玉米抗旱、高产提供基因资源、理论支持与种质材料。

玉米是重要的粮食、经济及饲用作物，但其生长与产量均受干旱胁迫严重制约。除常规育种外，转基因技术是提高玉米干旱抗性的有效方式。MAPK级联途径是真核生物体内一种重要的信号转导方式，参与环境胁迫应答等众多生物学过程。但玉米中与干旱抗性密切相关的MAPK途径仍未阐明。通过转录组分析，我们分离鉴定了玉米中受干旱胁迫明显诱导的基因ZmMAPKKK19，并证实该基因与玉米干旱抗性密切相关。本项目以ZmMAPKKK19为研究对象，通过转基因技术、正向与反向遗传学、生理生化指标检测等方法，阐释玉米ZmMAPKKK19介导的完整MAPK信号转导途径(ZmMAPKKK19-ZmMAPKK10-ZmMAPK2-ZmCP11)及其调控干旱抗性的分子机制,并创制抗旱性明显增强的玉米自交系三份，为实现玉米抗旱、高产提供基因资源、理论支持与种质材料。