生物泥浆反应器处理土壤多环芳烃过程中共代谢机理研究

Polycyclic aromatic hydrocarbons (PAHs) pollutants are the key limiting factor restricting bioremediation of soil contaminated with petroleum hydrocarbons. PAHs with high molecular weight are difficult to be utilized by microbe as carbon and energy sources, the degradation of these PAHs are usually conducted in the pattern of cometabolism. In this project, the mechanism of cometabolism for PAHs with high molecular weight was studied; substrates of metabolizing for typical PAHs with high molecular weight were screened; the cometabolizing processes of PAHs pollutants in a bioslurry reactor uses for treatment of contaminated soils were described. In this project, we also proposed the principles of carbon and gnergy sources for cometabolizing; set up the describing methods of oxygenase and lipase to cometabolizing processes; revealed regularity of coordinating effects of microbe and carbon source in degradation of PAHs in a certain degree..It was found that the introduced strains were difficult to compete with indigenous strains without sterilization of soil. To keep the survival ability of introduced strains, technology of cells immobilization using for remediation of contaminated soils was studied and applied in bio-slurry reactor. The effects of pollutants concentrations and amounts of inoculating on PAHs removals using immobilized bacteria and fungi cells were probed respectively. The research results showed that the removing rates of phenanthrene and pyrene were increased by 2 times using cells immbilization. This will provide the application of high effective strains with technical supports. .The research achive ment has been successfully applied in the bioremediation of soil contaminated with petroleum hydrocarbons in Liaohe Oil Field.

系统研究生物泥浆反应器处理土壤中多环芳烃过程的共代谢机理，通过高分子量多环芳烃共代谢底物的筛选，提出共代谢碳源优化理论；建立和氧酶对共代谢过程的定量描述方法，揭示微生物与共代谢碳源在多环芳烃降解中的协同作用效应；建立共代谢反应动力学模型与理论体系，优化生物泥浆反应器的运行条件，为多环芳烃污染土壤的生物净化提依据。.