尖孢镰刀菌番茄专化型乙酰化分泌蛋白FolSVP1功能解析

Fusarium wilt disease of tomato is a difficult vascular disease caused by Fusarium oxysporum f. sp. lycopersici. It is widely distributed in the world and is one of the most important soil-borne phytopathogens. To control the Fusarium wilt, research on the interaction mechanism between pathogenic effector proteins and host has become a hot topic in plant pathology. In this proposal, we identified a virulence-related secreted protein FolSVP1 (F. oxysporum f. sp. lycopersici secreted virulence-related protein1) with acetylation modification in the 167 lysine (K) site. The FolSVP1 was localized in nuclear and play an important role for the fungal pathogenicity. The function of FolSVP1 and its acetylation modification will be further investigated using gene knock out and complementation and K point mutation methods. Meanwhile, our study will further focus on screening its interaction proteins by yeast two-hybrid and Co-IP/LC-MS techniques, and characterize the roles of FolSVP1-interacting proteins in plant immunity using CRISPR-Cas9 system. Our study will finally help to understand how the plant acts in response to F. oxysporum infection by analyzing the function of FolSVP1, and likely provide new gene recourses for genetic engineering of crop resistance against pathogenic fungi.

番茄枯萎病是由尖孢镰刀菌番茄专化型（Fusarium oxysporum f. sp. lycopersici）侵染引起的一种防治困难的土传维管束病害，以枯萎病防治为核心开展的效应蛋白与寄主互作机制研究已成为植物病理学界的研究热点。本项目从尖孢镰刀菌番茄专化型分泌蛋白组中鉴定到一个167位赖氨酸（K）乙酰化修饰的新型外泌蛋白FolSVP1，该蛋白定位到植物细胞核中，并与尖孢镰刀菌的致病力密切相关。本项目拟通过基因敲除、回补、K点突变等方法解析FolSVP1及其乙酰化修饰在致病过程中的生物学功能；利用酵母双杂、免疫共沉淀等方法鉴定寄主中的互作靶标蛋白；利用CRISPR/Cas9技术突变靶标蛋白，通过番茄抗病反应变化揭示寄主应答机制。本项目将解析FolSVP1及其乙酰化修饰在尖孢镰刀菌致病过程中的功能，这对于加深理解尖孢镰刀菌的致病机理和构建该类病害的高效防控策略具有重要的理论指导意义。

番茄枯萎病是由尖孢镰刀菌番茄专化型（Fusarium oxysporum f. sp. lycopersici）侵染引起的一种防治困难的土传维管束病害，以枯萎病防治为核心开展的效应蛋白与寄主互作机制研究已成为植物病理学界的研究热点。本项目从尖孢镰刀菌番茄专化型分泌蛋白组中鉴定到一个167位赖氨酸（K）乙酰化修饰的新型外泌蛋白FolSVP1，该蛋白定位到植物细胞核中，并与尖孢镰刀菌的致病力密切相关。本项目通过基因敲除、回补、K点突变等方法解析FolSVP1及其乙酰化修饰在致病过程中的生物学功能；利用酵母双杂、免疫共沉淀等方法鉴定寄主中的互作靶标蛋白；利用CRISPR/Cas9技术突变靶标蛋白，通过番茄抗病反应变化揭示寄主应答机制。本项目解析FolSVP1及其乙酰化修饰在尖孢镰刀菌致病过程中的功能，对于加深理解尖孢镰刀菌的致病机理和构建该类病害的高效防控策略具有重要的理论指导意义。