基于光受体基因的南亚热带森林群落谱系结构研究

Analyzing phylogenetic structure with molecular evolution methods is an important way to study community assembly. However, the most common used community phylogenetic analyses by DNA barcode sequences have its limitation. These DNA sequences are conserved in evolution thus do not correspond to environmental changes that cannot directly reflect the relationship between them. As a result, we develop a method of analyzing community phylogenetic structure by functional genes. In this study, we apply this approach on the south subtropical forest 20-ha Dinghushan forest dynamics plot by using the sequence of plant light receptors phytochromes, cryptochromes and phototropins to construct the phylogenetic trees. Then, we analyze the phylogenetic structures with time, spatial and habitat scales. In addition, we compare the generated results to the DNA barcode results, and assess the feasibility of this method. By exploring the community assembly mechanism of south subtropical evergreen broadleaf forest, we aim to provide introduction to protection and utilization of in situ biodiversity.

通过分子进化研究群落谱系结构对了解群落构建具有重要意义。但目前普遍采用的DNA条形码序列（DNA barcode sequences）进行物种进化关系度量具有一定局限性。这些DNA序列在进化上保守，受环境变化影响不大，无法真正反映生物进化和环境变化之间的关系。为此，以南亚热带鼎湖山20 ha样地为研究地点，本项目通过功能基因（光受体基因的光敏色素、隐花色素和向光素序列）构建物种谱系树，分析不同时空尺度和生境条件下的群落谱系结构，并对比利用DNA条形码所得的结果，探讨该技术的可行性。在结果的基础上，深入探讨南亚热带常绿阔叶林群落构建机制，为当地生物多样性保护和利用提供指导。