人类基因功能分析模式系统的建立

The accomplishment of human genome project (HGP) will make the analysis of gene function becomes the core of research in post-genome era. The Drosophila becomes an excellent model organism for genetics, analysis gene function(s) and many biotechnologies arise. We constructed Gal4-UAS system, which is a good system to express exogenous gene and analyze its function. Firstly, we constructed the expression vectors (RP2-pUAST, RP2(del6)-pUAST, SNF1A-pUAST). Secondly, we transgened these plasmids into fly by microinjection. Finally, we balanced the transgenic flies and crossed the transgenic flies with Gal4 lines. .S2 cell transfection, PCR, Western blot and Fluorescence analysis detected the expression of these transgenes. These results show that human gene can express at fly. And we make sure the system of fly can analysis human gene's function by large scale.

以果蝇为模式动物,建立分析人类基因功能的研究系统.这个研究系统可以在组织水平和个体水平对人类基因功能进行分析,适用于有毒和致死作用的基因.以及在发育过程中表达的基因.使在技术上和伦理上都受到约束的大量人类基因的功能分析成为可能.