花生组蛋白去乙酰化酶AhHDA1影响的关键ABA信号通路分子（AIKASPGs）筛选及作用机制研究

Plant hormone abscisic acid is involved in regulating cell stress defense reaction. In recent years, it has been found that histone acetylation/deacetylation is involved in ABA signal transduction pathway, but the mechanism is not clear. In the previous experiment, the group found that peanut histone deacetylase AhHDA1 inhibited the expression of protein kinase AhSnRK1-like gene and transcription factor AhAREB1 gene in ABA signal transduction pathway. However, it is not clear that which are the major molecules AhHDA1 plays a role in ABA signaling pathway. This project mainly solves the following problems: 1.Identify the key molecules (AIKASPGs) that influence AhHDA1 on the ABA signal transduction pathway; 2.Define the gene function of AIKASPGs and its role in ABA signaling pathway;The role of AhHDA1 in ABA signaling pathway is deeply understood. 3.The function mode AhHDA1 influenced AIKASPGs. The results of the study will test AhHDA1 involved in ABA signal feedback inhibition of new ideas, but also from the histone acetylation modification adjustment Angle of ABA signaling pathway provide a new direction for research to improve crops drought resistance.

植物激素脱落酸参与调控细胞胁迫防御反应。近年来研究发现组蛋白乙酰化/去乙酰化修饰参与ABA信号转导途径，但作用机制不清楚。课题组在前期实验中发现花生组蛋白去乙酰化酶AhHDA1抑制ABA信号转导通路上蛋白激酶AhSnRK1-like基因和转录因子AhAREB1基因表达。但是不明确AhHDA1在信号通路中发挥作用的主效分子。本项目拟采用生物信息高通量筛选、植物遗传转化和双荧光素酶报告基因检测等关键技术解决以下问题：1.明确ABA信号转导通路上AhHDA1影响的关键分子（AIKASPGs）；2.明确AIKASPGs的基因功能及在ABA信号通路中的作用；3.AhHDA1影响AIKASPGs的作用方式。从而深入认识AhHDA1在ABA信号通路中的作用。研究结果将检验AhHDA1参与ABA信号反馈抑制的新设想，同时也从组蛋白乙酰化修饰调节ABA信号通路角度为研究提高农作物抗旱性提供一个新方向。

植物激素脱落酸参与调控细胞胁迫防御反应。近年来研究发现组蛋白乙酰化/去乙酰化修饰参与ABA信号转导途径，但作用机制不清楚。课题组在前期实验中发现花生组蛋白去乙酰化酶AhHDA1抑制ABA信号转导通路上蛋白激酶AhSnRK1-like基因和转录因子AhAREB1基因表达。但是不明确组蛋白乙酰化影响ABA信号通路的主效分子。本项目采用生物信息高通量筛选、植物遗传转化和启动子活性检测等关键技术：1.明确了ABA信号转导通路上组蛋白乙酰化修饰影响的关键基因为AhPDCP37；2.明确了AhPDCP37基因在萌发中的作用及在干旱和ABA信号通路中的作用；3.同时发现组蛋白去乙酰化酶抑制剂作用下降低了干旱条件下AhPDCP37基因启动子的活性。研究深入认识了组蛋白乙酰化在ABA信号通路中的作用。研究结果证实了组蛋白乙酰化作用影响ABA信号关键信号分子从而参与干旱反馈抑制的设想，同时也从组蛋白乙酰化修饰调节ABA信号通路角度为研究提高农作物抗旱性提供一个新方向。