羟基氨基喹啉衍生物手性光散射探针试剂的制备及其手性识别应用研究

Chiral enantiomers dominated natural wonders and the mysteries of life. Only the chiral environment in life organization could open up the chiral recognition and activation of the chirality naturally but human intervention in the chiral recognition by the special analytical means of combining high sensitivity and high selectivity, especially without separation firstly direct measurement and characterization of kinds of biological activity of different enantiomers. Resonance Rayleigh scattering (RRS) spectrum is a kind of high sensitivity and simple analysis method，and the RRS spectra with high sensitivity had the organic dyes, nanoparticles or quantum dots for light scattering probe reagent, not only enhanced its light scattering, improved the sensitivity, increased the affinity of reactants and biological macromolecules, could also effectively improve its selectivity. The project ideas are to put forward the preparation and application of the chiral light scattering probe reagent, more directly and effectively in chiral recognition of chiral enantiomers. Main research: (1) to confirm the action mechanism and sorting and expand research of hydroxyl, amino quinoline derivatives for chiral light scattering probe reagent; (2) to explore the synthesis and preparation of hydroxyl amino quinoline derivatives for chiral light scattering probe reagent; (3) to develop the new method of RRS spectral analysis dominated by chiral light scattering probe reagent of organic enantiomers in chiral recognition, further to establish a new analysis method for simultaneously determination of chiral enantiomers without separation firstly.

手性对映体主导着自然的奇妙和生命的奥秘。生命组织中的手性环境能自然开启手性识别和激活手性，而人为介入的手性识别需要高灵敏度和高选择性相结合的分析手段，尤其是不经分离而直接测量表征对映体不同的生物活性。共振瑞利散射(RRS)光谱是一种高灵敏度且简便易行的分析方法，以有机染料、纳米粒子或量子点等作为光散射探针结合高灵敏度的RRS光谱，不仅能增强光散射，提高灵敏度，增加生物大分子作用的亲和力，还能有效提高共振光散射光谱的选择性。本项目的创新设想提出手性光散射探针试剂的制备和应用研究，更直接有效地对手性对映体进行手性识别。主要研究：①确定羟基氨基喹啉类衍生物手性光散射探针试剂的作用机理和分类筛选及其拓展研究；②探索羟基氨基喹啉类衍生物手性光散射探针试剂的合成制备；③发展以手性光散射探针试剂为主导的识别手性对映体的RRS光谱分析新方法，进一步拓展确立不经分离而同时测定手性对映体的新分析方法。

围绕建立“不经分离而同时测定手性对映体”的新分析方法的创新设想，持续探索以高灵敏度的共振瑞利散射（RRS）光谱法对有机对映体的手性识别的方法研究， 需要寻求高选择性的新技术方法。于是在大量的实验基础上，提出手性光散射探针的概念，旨在与RRS方法结合以提高方法的选择性，并付诸实施手性光散射探针试剂的合成制备以及手性选择性分析的推广应用。本次项目应完成①确定羟基氨基喹啉类衍生物手性光散射探针试剂的作用机理和分类筛选；②探索羟基氨基喹啉类衍生物手性光散射探针试剂的合成制备等主要研究内容外，还拓展贵金属纳米粒子、手性功能化的量子点和碳点，以及筛选手性适配体和氧化石墨烯等高选择性的新技术，更主要实现③发展以手性光散射探针试剂为主导的识别手性对映体的RRS光谱分析新方法。研究表明针对不同的手性对映体反应体系， 以高灵敏和高选择性的新方法下凸显RRS光谱的特征变化和信号差异，再结合多种分子光谱方法的验证，辅之“同原射线计量分析”等化学计量学手段，可实现不经分离而同时测定手性添加剂、手性对映体药物或农药的简捷新方法。这不仅扩展和深化RRS光谱的新分析应用，而且拓展了有机对映体的手性识别的新途径。