牙周病病原菌DNA探针的研制及其应用基础研究
基本信息
结项摘要
选择与牙周炎关系最为密切的四种可疑牙周致病菌：牙龈卟啉菌、放线共生放线杆菌，中间型普及菌和具核梭杆菌作为研究对象，提取染色体DNA，随机克隆入载体PUC19，转化大肠杆菌JM109，从中筛选出能与同种细菌染色体DNA进行特异性杂交而不与其它相近的细菌染色体DNA交叉杂交的克隆，作为该种细菌特异性的DNA探针。本课题筛选出PZJ-AaI，含2.1kb放线共生放线杆菌染色体DNA片段，PZJ-PgI，含1.8kb牙龈卟啉菌染色体DNA片段，PZJ-PiI，含2.7kb中间型普反菌染色体DNA片段，PZJ-FnI，含1.3kb具核梭杆菌染色体DNA片段，该四个克隆各与同种细菌染色体DNA呈特异性杂交反应，其敏感性在地高率标记下为10ng染色DNA，可以作为这中种可疑牙周病原菌的DNA探针。
项目摘要
项目成果
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数据更新时间：2023-05-31
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