蛇足石杉离体培养物形态异化与累积石杉碱甲的相关性探究

Huperzine A (Hup-A) supplement is known to be the most effective medicine treating Alzheimer's disease. Hup-A is a natural compound derived mainly from Huperzia serrata plant. Insufficient supply of Huperzia serrata plant has become a major obstacle for new drug development. Through the previous studies,the applicant has established the platform for in vitro culture of the thallus clone of Huperzia serrata, In the present study, cytological characteristics of Huperzia serrata culture during its in vitro growth and development will be studied, and its laws of biochemical metabolism related to Hup-A synthesis and accumulation, and construct the theoretical basis for in vitro culture to Hup-A development process and associated molecular cell biological principles will be clarified. This study will provide not only theoretical and technical support for in vitro growth of Huperzia serrata thallus and subsequent Hup-A production, but the basis for further study on biosynthesis pathway of natural Hup-A.

石杉碱甲(Huperzine A, Hup-A)制剂是国内外治疗老年性痴呆症的最有效药物，其主要药源-自然蛇足石杉资源的匮乏是限制相关新药研发的最大瓶颈。申请者通过前期工作，已建立了蛇足石杉离体培养物无性繁殖系平台。本项目拟在离体培养条件下，研究蛇足石杉培养物中器官发生过程的组织细胞特点及其生化特征；研究不同形态的培养物与其合成积累Hup-A的相关性；研究培养物形态与其细胞生物学基础、生化代谢规律、次级代谢合成积累Hup-A功能的内在联系，从而建立培养物筛选标志的方法原理，揭示蛇足石杉离体培养物形态建成及其异化与产Hup-A的相关机理。本项目的实施，将为蛇足石杉的人工离体培养及天然Hup-A的生物合成途径的阐明提供基础资料和理论依据。

石杉碱甲(Huperzine A, Hup-A)制剂是国内外治疗老年性痴呆症的最有效药物，其主要药源-自然蛇足石杉资源的匮乏是限制相关新药研发的最大瓶颈。本课题应用现代生物技术科学中的多种方法，对蛇足石杉叶状体在不同基质上，离体培养物形态异化与次生代谢物石杉碱甲的含量变化的相关机理进行了研究。研究内容包括蛇足石杉培养物中器官发生过程的组织细胞特点及其生化特征；不同形态的培养物与其合成积累Hup-A的相关性；培养物形态与其细胞凋亡分子特征、生化代谢规律、次级代谢合成积累Hup-A功能的内在联系。同时对蛇足石杉不同形态的离体培养物中过氧化物酶同工酶谱进行了探索性研究。这些研究工作，取得了一些有意义的结果和经验,它将为蛇足石杉的人工离体培养及天然Hup-A的生物合成途径提供基础资料和依据。