荧光印迹复合材料的制备与选择性识别、检测环境中磺酰脲类除草剂残留的应用基础研究

Sulfonylurea herbicides are a class of the largest amount herbicide in the world and play a key role in the agricultural production. However, sulfonylurea herbicides are harmful for crop and human. So, it is extremely important to monitor sulfonylurea herbicide residues concentration during the process of agricultural production. We will explore the specific selective recognition mechanism of molecularly imprinted polymers, and investigate fluorescent detection on the special behavior of the sulfonylurea herbicide residues by combining fluorescent high sensitive detection technique with high selective recognition of molecular imprinting technique. With the help of selective recognition site and sensitive detection of fluorescent molecularly imprinted polymers, we will improve the accuracy and precision in the process of detecting sulfonylurea herbicides. By using the advanced characterization,law of bonding and the interfacial chemistry will be investigated between the target molecule and fluorescent molecular imprinted polymers, and the equilibrium，kinetics and thermodynamics of selective recognition and detection will be discussed in detail. In the fluorescence detection, the relationship will it is will be illustrated that between fluorescence intensity changes and qualitative detection. At last, a rapid, sensitive and accurate detection method will be established.

磺酰脲类除草剂是世界上用量最大的一类除草剂，在农业生产中起着举足轻重的作用。但对下茬作物及人体都有较大的危害，因此监测农业生产环境中的磺酰脲类除草剂的浓度残留显得尤为重要。本项目拟将荧光高灵敏检测与分子印迹高选择性识别相结合，建立荧光印迹复合材料快速、灵敏、准确的荧光识别、检测体系，与实验结果相结合探讨分子印迹聚合物的特异选择性识别机理及荧光检测磺酰脲类除草剂的特殊行为。研究中以荧光分子印迹聚合物选择性识别检测磺酰脲类除草剂为主线，以聚合物的制备方法和性能优化为关键控制点，通过荧光材料定向合成和印迹位点的识别程度提高检测磺酰脲类除草剂的准度和精度。在此基础上，深入研究荧光分子印迹聚合物与靶分子之间的界面化学及成键规律；探究荧光分子印迹聚合物选择性识别、荧光检测机理；揭示荧光强度变化与磺酰脲类除草剂残留的定量、定性的关系；建立选择性快速、灵敏、准确检测磺酰脲类除草剂的新方法。

磺酰脲类除草剂是世界上用量最大的一类除草剂，在农业生产中起着举足轻重的作用。但对下茬作物及人体都有较大的危害，因此监测农业生产环境中的磺酰脲类除草剂的浓度残留显得尤为重要。本项目将荧光高灵敏检测与分子印迹高选择性识别相结合，建立荧光印迹复合材料快速、灵敏、准确的荧光识别、检测体系。项目内容中利用二氧化硅，四氧化三铁，埃洛石等载体材料制备核壳型、外包型和内嵌型材料，提高了基质材料的表面效应、耐酸碱性差，减少磁性粒子泄漏；本项目中还制备了烯丙基、6-羧基和异硫氰酸酯基修饰的荧光素衍生物，并将其接枝在硅基和磁性基材料表面获得硅基和磁性荧光复合材料；采用沉淀聚合和ATRP等聚合方式，制备了多种硅基和磁性基荧光分子印迹材料。通过实验系统研究了识别平衡、动力学、热力学、靶向识别的影响因素和规律；利用仪器表征获得了模板分子与硅基和磁性基荧光分子印迹材料结合位点间的识别机制。建立了硅基和磁性基荧光分子印迹材料选择性识别分离与测定磺酰脲类除草剂残留的方法，实现多种样品如河流沉积物、河流水、水生动物和污水中痕量/超痕量磺酰脲类除草剂残留快速、高效选择性分离识别与测定。