中间锦鸡儿非生物胁迫相关NAC转录因子筛选与鉴定

Drought, cold and saline undermine agricultural production seriously, but genetic engineering improves the stress resistance of crop substantially. Caragana intermedia, which is widely distributed in the northwest China, is an important forage bush belonging to the legume family. It has a great potential for research on stress-resistant genes owing to its strong tolerance to various stresses. In order to study its resistance mechanisms to drought and to screen drought responsive genes, a transcriptome of C. intermedia under drought stress was constructed in previous work. Because of the importance of the NAC genes in stress responses, we plan to clone the abiotic stress-induced NAC genes from C. intermedia and their promoters, and analyze mRNA expression profile of these NAC genes under various abiotic stresses (such as drought, saline and cold), in different organs and at different development stages. Transgenic Arabidopsis thaliana or Medicago truncatula will be obtained to reveal their function and molecular mechanisms in resistance to abiotic stresses. This study will lay the foundation of applying NAC gene of C. intermedia to genetic engineering of crop's stress resistance and tree breeding.

干旱、冷冻和盐碱等非生物胁迫严重危害农业生产，植物抗逆基因工程是提高作物抗逆能力的重要手段。中间锦鸡儿是西北荒漠地区重要的豆科灌木，耐逆性强，挖掘抗逆基因的潜力巨大。本课题组对脱水处理的中间锦鸡儿进行了转录组深度测序，获得了大量抗逆相关基因。因为NAC转录因子在植物抗逆生理中有着重要作用，本研究组拟筛选、克隆中间锦鸡儿抗非生物胁迫关键的NAC基因及其启动子，进而分析这些基因在干旱、冷冻和高盐等逆境胁迫条件下以及在不同生育期、不同器官中的表达情况。通过转基因拟南芥或蒺藜苜蓿，研究这些基因的生理功能，揭示NAC基因参与植物生长发育或抗逆的分子机制，评价其应用价值和潜力，为中间锦鸡儿NAC基因在作物抗逆基因工程和林木育种中的应用奠定基础。

干旱、低温和盐碱等非生物胁迫严重危害农业生产，植物抗逆基因工程是提高作物抗逆能力的重要手段。中间锦鸡儿是西北荒漠地区重要的豆科灌木，耐逆性强，挖掘抗逆基因的潜力巨大。本课题组对脱水处理的中间锦鸡儿进行了转录组深度测序，获得了大量抗逆相关基因。前人的研究表明，NAC转录因子在植物抵抗逆境胁迫中起着重要作用，本研究组拟筛选、克隆中间锦鸡儿抗非生物胁迫关键的NAC基因及其启动子，进而分析这些基因在干旱、低温和高盐等逆境胁迫条件下以及在不同发育阶段、不同器官中的表达情况。通过转基因拟南芥或蒺藜苜蓿，研究这些基因的生理功能，揭示NAC基因参与植物生长发育或抗逆的分子机制，评价其应用价值和潜力，为中间锦鸡儿NAC基因在作物抗逆基因工程和林木育种中的应用奠定基础。.项目按申请书和计划任务书实施，筛选克隆了CiNAC1、CiNAC2、CiNAC3、CiNAC3-3、CiNAC4、CiNAC6、CiNAC8、CiNAC038、CiNAC042、CiNAC071、CiNAC100和CiATAF1等12个中间锦鸡儿响应非生物胁迫的关键 NAC 转录因子编码基因，开展了其在植物抵抗干旱、冷冻和高盐等非生物胁迫中的生物学功能研究。阐明了CiNAC1、CiNAC038、CiNAC042、CiNAC071和CiATAF1等5个基因的抗逆分子机制及参与非生物胁迫应答的调控网络，发表学术论文6篇。在中间锦鸡儿瞬时表达系统和特异启动子领域申请国家发明专利3项。