宿主干扰素效应因子IFI35在猪流感病毒感染中的功能研究

Swine influenza viruses have important public health significance.Host cells can express a series of interferon and inflammatory cytokines after swine influenza virus infection. The interferon play antiviral functions mainly through its downstream effectors. IFI35 is a newly interferon induced effector, however, its biological function especially in the mechanism of regulating viral infection is unclear. This study analyzes the changes of IFI35 expression and subcellular localization during swine influenza virus infection.We also interested its function in swine influenza virus replication, and its interaction with the virus nonstructural protein NS1. NS1 is well known as an interferon antagonist and it can bind with RIG-I during influenza virus infection. The aim of this study is to investigate the role of IFI35 in regulating RIG-I-mediated IFNβ activation pathway during swine influenza virus infection.This study can provide the molecular mechanism of IFI35 in the regulating host cells response to swine influenza virus infection and enrich people's awareness of the function of interferon-induced effectors during anti-virus process.

猪流感病毒具有重要的公共卫生学意义，猪流感病毒感染后诱导宿主干扰素及炎性因子表达，干扰素的抗病毒机制主要通过其下游诱导效应因子而发挥作用。IFI35属于新的干扰素诱导效应因子，其生物学功能尤其是其在病毒感染过程中的作用机制尚不清楚。本研究拟分析干扰素诱导效应因子IFI35在猪流感病毒感染后的表达变化、亚细胞定位变化、对病毒复制的功能分析、与流感病毒NS1蛋白的相互作用分析以及在调控RIG-I介导的IFNβ激活通路上的功能分析，揭示IFI35在调节宿主流感病毒感染的应答反应与分子机制，丰富人们对干扰素效应因子在抗病毒感染过程中的功能认知。