纳米材料分子印迹微柱富集/毛细管电色谱高效分析痕量β2-受体激动剂的研究

For improving the efficiency and specific identification of molecular recognition on monolith, the researchs on new nano materials of molecularly imprinted monolithic column and capillary electrochromatography (CEC) on-line identification enrichment separation of the new mode ould be done by using β2- agonists as the representative object. By means of the surface modification of nano materials on the skeleton of monolith and introducing molecular imprinting technology, a desired functional monolith will be prepared. Numbers of active sites for highly specific affinity chromatography are expected, and specific identification of β2- agonists on the nano materials of molecularly imprinted monolithic column will be also obtained. Through the field accumulation enrichment and high specific adsorption, a new nano material molecularly imprinted micro column enrichment-CEC efficient analysis technique is prepared. The problems existing in the traditional enrichment mode of monolith, such as small column capacity, large sample consumption and zone broadening, will be well avoid. It is favorable for the highly specific recognition, efficient enrichment and rapid separation of traceβ2- agonists. Besides, by using the sensitive electrochemical detection （ECD）method, CEC-ECD hyphenated technology would be established. It is highly sensitive and competent for the trace analysis of β2- agonists. Finally, international journals 3-4 research paper and 1-2 patents will be expected.

以β2-受体激动剂为研究对象，从功能材料开发和联用技术着手，创新研发新型纳米粒子分子印迹整体柱及毛细管电色谱在线识别富集、分离的新模式，致力于新型整体柱高效富集、特异识别的研究。项目将纳米粒子引入电色谱整体柱，在高相比的纳米粒子表面修饰分子印迹聚合物，形成大量的β2-受体激动剂高特异性识别作用位点，大幅提高整体柱的识别特性，研发出特异识别的高相比分子印迹整体柱；联用电色谱高效分析技术，建立集电场堆积富集、高相比特异性吸附的电色谱分离为一体的新型纳米粒子分子印迹微柱富集-毛细管电色谱电化学高效分析技术，解决常规整体柱分子识别作用相比较小、富集效率较低、样品富集区带展宽明显等问题，建立痕量β2-受体激动剂的特异性识别和高效分析技术。在此基础上，集成电化学柱上检测模式，实现生物样品中痕量β2-受体激动剂的纳米粒子分子印迹微柱富集-毛细管电色谱电化学高效分析。拟发表论文3-4篇、申请专利1-2项。

从纳米聚合整体材料开发和高灵敏度检测技术着手，研究应用整体聚合材料在柱修饰改性和溶胶-凝胶技术，将金纳米粒子、环糊精改性纳米氧化铁颗粒引入电色谱聚合材料，制备高相比的纳米粒子修饰杂化硅胶聚合材料；优化流动相中修饰试剂浓度、缓冲盐组成、缓冲溶液浓度、pH值、有机相含量等参数对β-受体激动剂的影响，确定五种β-受体激动剂最佳分离参数，在8.0 min实现对五种β-受体激动剂的高效分离。同时，结合电化学安培检测技术，优化分离检测参数，建立克伦特罗对映体的高灵敏度特异性分离检测模式。此外，基于场放大进样协同色谱区带压缩效应及大体积在线富集方法，开展了玉米赤霉醇类激素pCEC富集效果的影响分析和作用机制研究，与传统非富集进样方式相比，灵敏度提高了约3个数量级。此方法弥补了通用型紫外检测器灵敏度不足的缺陷，为简便的加压毛细管电色谱联用技术对多组分物质实现柱上富集及高灵敏分析提供了良好的技术支持。已在国内外刊物发表论文7篇，授权国家专利9项，培养研究生4人。