微生物的基因转化和固体废弃物处理

Several species of cellulose degrading fungi were screened and obtained from natural enviroment and other research institutes.Their activities of cellulase and the ability of degrading cellulose were studied.The Trichoderma viride AS 3.3711 was selected as the target strain for the cloning of cellulase genes. For cloning of the main cellulase genes of Trichoderma viride , RT-PCR and PCR were performed . The cDNA genes (EgⅠ,EgⅢ,EgⅤ,Cbh Ⅱand BglⅡ) and DNA genes (EgⅠ,EgⅢ and EgⅤ) were cloned and verified by DNA sequencing. Then these genes were constructed on the yeast expression vector pYES2 which had a high active promoter . Further more, two strains of yeast were selected as the host strains of the expression of these genes. This was the first time that obtained almost all main cellulase genes of Trichoderma viride in China, which made a good foundation of the next step research.

在固体废弃物处理过程中筛选优秀的纤维素降解菌，分离重组该菌株的纤维素酶基因，利用基因工程手段，将这一微生物中高活性结束素酶基因，转入优秀的酒精发酵微生物-酿酒酵钢校米瞬煌宋孛富虻慕湍妇窍绻烫宸掀镏械南宋爻煞葑凭剿魃锛际醮砉烫宸衔锊⑹蛊渥试椿男峦揪缎路椒ǎ岣吖烫宸衔镒试椿健