大鼠颅内动脉瘤壁平滑肌细胞增殖活性的探讨

Intracranial aneurysm (IA) is the primary cause of subarachnoid hemorrhage (SAH), its lethal and disable ratio are very high.At present ,the therapies of IA are limited to clipping operation and intravascular treatment, there are very few researches about nonoperative treatments such as repair therapy for IA wall. We found the proliferation activity of vascular smooth muscle cell(VSMC) fuction in the research of the machenismm of aneurysms in rat.As the development of biological and gene therapy,it would be showed us to bring the hope to repair therapy of the ruptured IA wall.Vascular smooth muscle cell (VSMC) is the major component of artery wall, we found it has the ability of phenotype transition project.In our experiment,we found that in the process of intracranial aneurysm formation, it puts up strong proliferation activity and plasticity. Thus it can be seen that the research on proliferation of smooth muscle cell of blood vessel wall in the process of intracranial aneurysm formation will become the main directions to repair therapy for IA wall, while smooth muscle cell layer will be the key and breakthrough point in the repair therapy. In the experiment,we will establish rat model of intracranial aneurysm and investigate the proliferation activity of smooth muscle cell of intracranial aneurysm wall so that we can provide theoretical basis to repair therapy of human intracranial aneurysm in the future.

颅内动脉瘤是神经外科的常见病，具有很高的致残率和死亡率。目前动脉瘤仅限于外科手术夹闭和血管内介入治疗，对动脉瘤壁的修复等非手术治疗基本没有。在对动脉瘤发生机理的研究中，我们发现动脉瘤壁的平滑肌细胞具有增殖活性功能。结合目前生物治疗和基因治疗的发展，我们萌生了为颅内破裂动脉瘤壁修复治疗的设想。血管平滑肌细胞（VSMC）是动脉组成的主要部分，实验中我们发现VSMC具有表型转换能力，在颅内动脉瘤形成过程中，可表现出很高的增殖活性与可塑性，可见动脉瘤形成过程中血管壁平滑肌细胞的增殖将成为修复研究的主要方向，平滑肌细胞层也将成为修复的关键和突破点。本实验拟在建立大鼠颅内动脉瘤模型的基础上，对大鼠颅内动脉瘤壁平滑肌细胞增殖活性进行研究，目的在于增强VSMC的增殖活性，增加平滑肌层，为今后人颅内动脉瘤壁的修复治疗提供理论基础。

颅内动脉瘤（intracranial aneurysm, IA）具有自发性破裂出血的倾向，致残率和致死率都很高。目前，在临床上关于IA的治疗仅仅局限于手术和介入治疗，而对动脉瘤壁进行修复等非手术治疗几乎没有。在对IA发病机制的研究中，我们发现动脉瘤壁血管平滑肌细胞（vascular smooth muscle cell, VSMC）具有增殖活性功能，结合目前生物治疗和基因治疗的发展，我们萌生了为破裂与未破裂颅内动脉瘤壁进行修复治疗的设想。VSMC是动脉壁组成的主要部分，在IA形成过程中，可表现出很高的增殖活性与可塑性，可见IA形成过程中血管壁VSMC的增殖将成为修复研究的主要方向。因此，本实验通过建立大鼠颅内动脉瘤模型对大鼠颅内动脉瘤壁VSMC增殖活性进行探讨，目的在于增强动脉瘤壁VSMC的增殖活性，增加平滑肌层，为今后人颅内动脉瘤壁的修复治疗提供理论基础。