大麦条纹花叶病毒TGB1与单子叶植物抗病蛋白Bsr1相互作用的结构解析

Plant has evolved diverse defense mechanisms against pathogen infection, of which the resistance gene (R gene) mediated hypersensitive reaction (HR) is the most efficient pathway. In the process of gene-for-gene reaction, the specific recognition mechanism induces rapid cell death to inhibit viral cellular or systemic movement. This proposal aims to understand the interaction of TGB1 with Bsr1, respectively from Barley stripe mosaic virus (BSMV) and its monocot host Brachypodium distathyon, through solving the complex structure of the two proteins. This project will provide us new evidences of the molecular mechanisms responsible for the interaction between viruses and host plants.

植物在抵御病原物入侵时产生了严密的防御机制，其中由抗病蛋白（R蛋白）介导的防御机制最为有效。这种具有特异性识别的机制通过植物过敏性坏死反应从而有效地抑制病毒在寄主中的移动。本申请项目将通过单子叶植物中第一个抗病蛋白Bsr1与大麦条纹花叶病毒的多功能蛋白TGB1的复合物晶体结构解析，从结构生物学角度诠释TGB1和Bsr1蛋白相互识别的分子机制，以及关键氨基酸参与互作的作用方式，为深入理解病毒与寄主植物互作的分子机理提供新的结构生物学依据。