P2X1受体介导的小胶质细胞激活和凋亡机理的研究
基本信息
结项摘要
我们的实验发现小胶质细胞(MG)表达P2X1、P2X4和P2X7受体，ATP可能通过P2X1受体参与MG的激活与凋亡。本课题将进一步证明ATP通过P2X1受体激活并诱导MG凋亡；以P2X1-HEK293细胞为模型，证明ATP通过P2X1受体诱导细胞凋亡；用广谱磷酸化抗体与ATP和P2X1受体拮抗剂等处理的MG和P2X1-HEK293细胞的蛋白样本相互作用，应用凝胶PA、SDS-PAGE电泳，质谱等技术以期找到与MG激活和凋亡相关的信号分子，查明其信号转导途径；在脑外伤模型中证明ATP通过P2X1受体参与MG激活和凋亡。活化的MG在脑外伤、缺血、炎症、退行性病等过程中起重要作用。病变局部及邻近区受损和受刺激的细胞激释放出大量ATP足以激活MG。激活的MG释放自由基、NO、和炎性物质等对局部细胞造成二次损伤。因此阻断ATP激活MG将减轻局部细胞的二次损伤，为辅助治疗上述疾病提供新的理论依据。
项目摘要
项目成果
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数据更新时间：2023-05-31
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