脱髓鞘疾病相关病理特征的非标记太赫兹光谱识别方法及其机制研究

Demyelinating disease is a group of process disease with complicated pathogenesis and multiple pathological characteristics in nervous system. It is important to develop more efficient tools for diagnosis and monitoring of the pathological course. Terahertz (THz) spectroscopy, a state-of-the-art research field, is promising in applications in diagnosis. Recently, we reported a novel approach based on terahertz spectroscopy combined with chemometrics to discriminate myelin deficit brain without exogenous labeling. We also demonstrated that THz spectroscopy is a useful cell analysis platform for non-invasive, label-free, real-time and in-situ monitoring cellular processes. These results suggested that THz spectroscopy is a new method for clinical diagnosis of multiple pathological characteristics in demyelinating diseases progression. This proposal aims to study the terahertz spectroscopies of demyelination, neuroniflammation, neuron damage, and remyelination in cuprizone induced mouse model of demyelinating disease. We will also compare the THz spectrum of nerve cells in vitro and myelin components with those of mouse brain to identify the markers of pathological characteristics. This project will provide a theoretical basis for the application of THz spectroscopy in biomedicine, and will provide a new insight of techniques for monitoring and diagnosis clinical demyelinating diseases.

脱髓鞘疾病是神经系统中一大类病因复杂、病理特征多的易复发、易致残疾病，现有技术难以同时实现无标记、定位且定性的病理分析，发展新型技术诊断监测整个病理进程具有重要意义。太赫兹（THz）光谱技术是一项新兴的光谱技术，近年来多项研究证明THz光谱技术在医学诊断领域有良好前景。前期我们发现THz光谱技术能无标记地识别脱髓鞘和正常脑组织，同时能实时原位监测活细胞生理过程，提示THz光谱技术有望用于脱髓鞘疾病进程中不同病理特征的监测诊断。因此，本项目拟采用Cuprizone诱导的脱髓鞘疾病进程动物模型，深入研究脱髓鞘疾病进程中脱髓鞘、神经炎症、神经元损伤、髓鞘再生等不同病理特征的THz光谱特性和识别方法，并从神经细胞生理过程、髓鞘主要生物分子两个层次对THz光谱识别不同病理特征的细胞分子机制进行研究，阐明脱髓鞘疾病进程相关的THz光谱标记物，为临床脱髓鞘疾病的早期诊断和长期监测诊断提供新的思路。

脱髓鞘疾病是神经系统中一大类病因复杂、病理特征多的易复发、易致残疾病,现有技术难以同时实现无标记、定位且定性的病理分析,发展新型技术诊断监测整个病理进程具有重要意义。太赫兹(THz)光谱技术是一项新兴的光谱技术,近年来多项研究证明THz光谱技术在医学诊断领域有良好前景。前期我们发现THz光谱技术能无标记地识别脱髓鞘和正常脑组织,同时能实时原位监测活细胞生理过程,提示THz光谱技术有望用于脱髓鞘疾病进程中不同病理特征的监测诊断。本项目利用脱髓鞘疾病之一帕金森猕猴模型，深入研究脱髓鞘疾病进程中神经元损伤病理特征的THz光谱特性和识别方法,并从神经细胞生理过程、髓鞘主要生物分子两个层次对THz光谱识别不同病理特征的细胞分子机制进行研究,发现了电刺激诱导神经冲动传导时太赫兹光谱的变化，同时明确了髓鞘蛋白MBP为脱髓鞘疾病进程相关的THz光谱标记物,为临床脱髓鞘疾病的早期诊断和长期监测诊断提供新的思路。