基于新型多功能磁性纳米仿生杂化材料的农药多残留快速分析研究

The serious hazard of pesticide residue to the environmental and human health is coming on global focus. However, the current detection technologies of pesticide residues are hard to meet our country’s urgent demand for the pesticide residues monitoring. To solve the bottleneck, a rapid pesticides multi-residue detection array based on the novel multifunctional magnetic biomimetic nanohybrid material is developed by combing the nanotechnology and molecular imprinting technology. Using organophosphorus pesticides as the model targets, a surface imprinted nanoshell over magnetic nanoparticles is fabricated to get the novel multifunctional magnetic biomimetic nanoparticles, which will possess high peroxidase-like catalytic activity, superparamagnetism and highly specific recognition ability for organophosphorus pesticides. Subsequently, the high-intensity peroxidase substrate is sieved or prepared, and then a rapid visual sensor array for simultaneous determination of six organophosphorus pesticides including diazinon, chlorpyrifos, parathion, parathion-methyl, fenitrothion and fenthion is established based on the magnetic biomimetic nanohybrid material-organophosphorus pesticides-substrate probe system. And this innovative research will provide a good foundation for the development of pesticide multi-residues rapid detection technologies, and also be very beneficial to improve the level of the quality and safety of agricultural products in China.

农药残留严重危害了人类健康和生态平衡，已成为全球焦点问题，然而现有的农药残留快速分析技术无法满足我国农药残留监管的需求。为解决这一瓶颈，本项目拟结合纳米技术、分子印迹技术、阵列检测技术开展基于新型多功能磁性纳米仿生杂化材料的农药多残留快速分析研究。以有机磷为模型农药，通过优化聚合体系和聚合方式，在磁纳米粒子表面制备有机磷的纳米印迹薄层，研究集超顺磁性、过氧化物催化活性、特异性识别能力于一身的多功能磁性纳米仿生杂化材料的制备方法。进一步，通过筛选或制备高响应的显色底物探针，利用有机磷农药、底物探针与多功能纳米杂化材料的竞争结合作用，开展二嗪农、毒死蜱、对硫磷、甲基对硫磷、杀螟硫磷、倍硫磷等6种有机磷农药残留的同时快速分析研究。这一研究工作将为发展农药多残留快速分析方法提供新的思路，有利于保障我国农产品质量安全。

以磁性多孔材料为载体，设计制备了对有机磷具有类特异性识别能力的磁性分子印迹纳米材料，建立了基于磁性多功能纳米识别材料的有机磷高效快速样品前处理方法，实现了对多种有机磷农药的同步分析。进一步地，以上述识别材料为识别元件，结合辣根过氧化氢酶标记技术和量子点荧光标记技术，利用目标分子与辣根过氧化物酶标记技术/量子点荧光标记的目标物类似物和识别材料的竞争结合作用，建立了有机磷农药的显色/荧光快速检测技术。此外，以分子印迹材料和金属有机骨架材料为基础，探索了纳米材料与农药分子的相互作用机制，筛选了对不同种类农药分子（新烟碱类、磺酰脲类、三嗪类等）具有类特异性识别能力的纳米识别材料，实现了多种农药高效分离富集及多残留检测。本研究为构建农药残留分析关键识别元件库具有指导意义，对农药多残留检测技术的开发具有较好通用性，有利于保障我国食品安全。