基于微流控芯片的光电化学生物传感新方法研究

The objective of this project is to construct a novel technique and method of microfluidic chip coupled with photoelectrochemical detection mode by combining the merits of microfuidic chip technique and photoelectrochemical method. The developed method will be used for the determination and applications of malignant hemopathy cells (K562) and their correlates (IL-6, MMP-2). The main contents of this project are as follows: (1) Design of the microfluidic device compatible with the photoelectrochemical detection mode; study of the device of microfluidic chip coupled with photoelectrochemical detection mode integration with some functional units such as cell culture chamber, reagent mixer, and concentration gradient generator; study of the binding of biorecognizing probe and the target and the interaction between the biorecognizing molecules and target in the microfluidic system. (2) Construction of the photoelectrochemical sensing interface with biorecognizing functions. The content includes the preparation of the nanomaterials, the construction of the photoelectrochemical sensing interface, and the study for enhancement of the sensitivity. (3) The application of the developed microfluidic chip coupled photoelectrochemical detection method in the sensitive analysis of malignant hemopathy cells and their correlates. The proposed method would provide a novel technique and method for fast, high throughput, high sensitive, and high selective detection of tumor cells and their correlates.

本项目拟将微流控芯片技术与高灵敏的光电化学检测手段相结合，构建微流控芯片光电化学检测联用新技术，开展对恶性血液病细胞（如K562细胞）及其相关物质（IL-6，MMP-2）的灵敏检测和应用研究。主要内容包括：（1）设计与光电化学传感模式相匹配的微流控芯片器件；根据不同研究对象，开展集成一定功能单元（细胞培养、试剂混合、浓度梯度生成）的微流控芯片光电检测器件研究；开展微流体系中生物识别探针与研究对象的结合研究以及生物识别分子与研究对象相互作用研究。（2）开展微流体系中具有生物识别功能光电传感界面的构建研究，包括纳米材料合成、光电传感界面的构建、光电响应灵敏度的提高等。（3）将发展的新方法应用于恶性血液病细胞及其相关物质的灵敏检测。为肿瘤细胞及其相关物质的快速、高通量、灵敏和选择性检测提供一种新技术和新方法。

项目围绕微芯片光电生物传感检测联用技术及生化分析应用研究这一课题，将承载一定分析功能的微芯片平台与光电生物传感技术相结合，通过共敏化、构建异质结等方法制备具有良好光电性能的纳米材料，结合抗体、适配体、小分子等识别元件构建光电传感界面，利用电子供体消耗/生成策略、酶辅助放大、激子-激元相互作用等信号放大手段，构建了一系列肿瘤细胞及相关标志物快速、灵敏、准确检测的新技术和新方法。同时，在项目进展过程中，根据研究工作实际有所拓展，开展了一些其他如电化学发光、毛细管电泳电化学发光/化学发光等生物分析新方法研究。项目的实施为生物分子与肿瘤细胞及相关标志物相互作用机制研究和肿瘤早期诊断提供了新方法和新技术。