褐腐真菌降解纤维素的机理研究

A special low-molecular-weight peptide named Gt factor, was isolated and purified from the extracellular culture of the brown-rot fungus Gloeophyllum trabeum via HPLC. It had high-affinity Fe3-chelating ability and could reduce Fe3+ to Fe2+; In presence of O2 , it could produce hydroxyl radical HO˙ with cellulosic materials as electron donors. Effects of Gt factor on cellulose degradation suggested that Gt factor could disrupt inter- and intra- hydrogen bonds in cellulose chains by HO˙-involved mechanism, and lead cellulose chains to depolymerization which brought about more reducing and non-reducing ends exposed, thus making cellulose accessible for further degradation. This pathway was quite different from the hydrolytic processes by cellulases and Gt factor might play an important role in the early stage of cellulose depolymerization by brown-rot fungi.

组建菌体—纤维素，生物电化学反应器。用循环伏安法研究密粘褶菌降解纤维素时的氧化还原电位变化。分离，纯化具电子传递功能的低分子螯合物（电子载体）。研究其氧化降解纤维素时的电子传递过程，由此构建可氧化性降解纤维素的生物电化学反应体系。可阐明证实并模拟褐腐真菌这一独特的纤维素降解机理，为经济有效的利用木质纤维材料开拓新途径。