TLR3配体预处理人脐带间充质干细胞对其治疗帕金森病的影响及机制研究

Human umbilical cord mesenchymal stem cells (hUC-MSCs) is a potential promising cell to treat Parkinson`s disease (PD). It is an urgent need to enhance its migration ability. The activated TLR3 could facilitate cell migration, but their effects on hUC-MSCs are still unknown. In this study, we will investigate the TLR3 expression in hUC-MSCs, and then observe its effects on the migration of hUC-MSCs. The effects of TLR3 on the related biological properties of hUC-MSCs will be explored. Finally, we will evaluate the therapeutic effects of TLR3 activated hUC-MSCs on Parkinsonian mice via CSF delivery, investigate the underlying mechanisms and trace the distribution of hUC-MSCs in the brain of PD mice. In this study, we will reveal the TLR3 effects on hUC-MSCs treating PD, for providing a new way to treat PD.

人脐带间充质干细胞（hUC-MSCs）对治疗帕金森病（PD）有很大的应用潜能，如何提高细胞迁移能力是需要解决的重要问题之一。新近研究发现TLR3受体的活化能够促进细胞迁移，但对hUC-MSCs迁移能力的影响未见报道。本研究首先检测TLR3在hUC-MSCs中的表达情况，观察其相应配体对hUC-MSCs迁移能力的影响，接下来探索TLR3对hUC-MSCs的生物学特性的影响，最后将TLR3配体预处理的hUC-MSCs通过枕骨大孔输注到PD小鼠脑脊液中，评价其疗效并探讨相关机制。本课题将系统的研究TLR3配体对hUC-MSCs功能的影响并为PD的治疗提供新方法。

炎症是帕金森病（PD）的重要发病机制，间充质干细胞（MSCs）具有抗炎作用和归巢性。本研究将大鼠骨髓间充质干细胞注射入PD小鼠脑脊液中，观察了MSCs对PD小鼠的疗效及机制。结果显示，MSCs提高了PD小鼠纹状体TH含量，改善其黑质部位TH阳性神经元数量及其前后肢步幅，抑制了其黑质部位的小胶质细胞的活化。我们的研究结果提示MSCs对PD小鼠有治疗作用，这一作用可能是通过MSCs的抗炎作用实现的。