卵黄DNA 的序列属性和功能的研究

Following the study of general characteristics of yolk DNA and yolk RNA, a plasmid library of yolk DNA of fertilized eggs was constructed. ss cDNA probes of yolk were obtained by a Dig-labelling procedure and used to screen 200 clones of the plasmid library. 16 clones hybridized with the ss cDNA probes, which indicates that at least part of yolk RNA was transcribed from the yolk DNA. The 16 clones were sequenced and the sequence comparisons with genetic databases revealed that 7 of them was highly similar to those from either Xenopus or other species. These genes encode metabolic enzymes and polyamine transport protein, respectively. So we can speculate that embryotic development may be directed by the yolk DNA. The other 9 clones appeared to be unknown in the genetic databases. They may be the superiovity.genes in yolk.

继已完成的卵黄DNA和卵黄RNA 的一般特性的研究将构建卵黄DNA文库和卵黄RNA的cDNA文库⒉庑虿糠挚寺Ｍü隚enBankTM数据库比较推测卵黄DNA和卵黄RNA的功能；寻找卵黄RNA欣醋月鸦艱NA的序列并重点探讨部分功能基因的表达状况。本研究有望揭示卵黄DNA的属性和功能并引发有关鸡胚早期发育和细胞增殖的新思考。