艾灸烟雾PM2.5对大鼠肺组织毒性阈值的多元线性研究

As one of the important effective factors of moxibustion therapy, Moxibustion Smoke is an integral part of traditional moxibustion therapy. However, by people’s enhancement of environment protection consciousness, the safety evaluation of Moxibustion Smoke has become a key factor for the survival and development of traditional moxibustion therapy. This project propounds a theory of Moxibustion Smoke’s space-time-concentration effection, takes PM2.5 of Moxibustion Smoke and male SD rats as the research objects, intends to study the PM2.5 content of Moxibustion Smoke by time、distance and concentration, establish the multiple linear regression model by the PM2.5 content of Moxibustion Smoke, explore the toxic effect on rats’ lung tissue by PM2.5 of Moxibustion Smoke and it’s mechanism of action by studying the male SD rats’ lung coefficient, lung tissue’s wet-dry specific gravity, bronchoalveolar lavage fluids’ compositions, phagocytic function of lung macrophage, pathomorphology of lung tissue, expression of TLR2、TLR4、NF-кB in lung tissue and the three-dimensional distribution of PM2.5 in lung tissue. Establish the multiple linear regression model for the toxic threshold dose that PM2.5 of Moxibustion Smoke works on the rats’ lung tissue, scientifically evaluate the Moxibustion Smoke PM2.5’s harmful threshold dose and non-toxic threshold dose, provide the experimental basis for the reasonable clinical Moxibustion Smoke control standards, in order to preferably inherit and develop traditional moxibustion therapy by improvement.

作为艾灸起效的重要因素之一，艾烟是传统艾灸疗法不可或缺的组成部分，但随着人们环保意识的增强，艾烟的安全性评价已成为传统艾灸疗法是否能继续存在发展的关键因素。本项目提出“艾烟时空浓度量效学说”，以艾烟PM2.5及雄性SD大鼠为研究对象，从时长、距离、浓度三方面对艾烟PM2.5含量进行研究，建立艾烟PM2.5含量的多元线性回归模型，并通过对不同浓度、时长艾烟干预下雄性SD大鼠肺系数、肺组织湿干比重、肺灌洗液成分、肺泡巨噬细胞吞噬功能、肺组织病理学、肺组织TLR2、TLR4、NF-кB表达、肺组织PM2.5三维分布形态的研究，探究艾烟PM2.5对大鼠肺组织的毒性作用及其作用机理，建立艾烟PM2.5对大鼠肺组织毒性阈值的多元线性回归模型，科学评估艾烟PM2.5对肺组织的有害阈剂量和非有害阈剂量，为指导临床合理制定艾烟控制标准提供实验依据，使传统艾灸疗法能在改进中谋发展，得到更好的传承和发扬。

艾灸是一种传统的中医疗法，但目前对艾烟的潜在毒性知之甚少。本课题研究了不同浓度的艾烟对大鼠肺组织的毒性作用，并探讨其作用机制。我们构建了不同浓度的艾烟大鼠模型，然后采用ELISA、IHC和RNA-seq等方法分析了不同浓度艾烟对大鼠肺组织的潜在影响。结果表明，低浓度的艾烟对大鼠肺部没有明显的毒性作用。相反，中、高浓度的艾烟显著增加了大鼠肺组织中的IL-6、TNF-a、NF-κB、TLR2和TLR4等炎症因子表达。RNA-seq分析表明，高浓度的艾烟可能会导致肺部缺氧相关通路激活而诱发肺部炎症反应，EGR1是艾烟引起肺部损伤的潜在靶基因，我们的研究将为今后艾烟的开发和临床应用提供研究基础。