金雀黄素对人子宫内膜雌激素受体调节及对子宫内膜病变防治机制研究

The incidence of endometrial lesions increased year by year, the age of onset is becoming younger and younger, which seriously affects the physical and mental health of women. Endometrial estrogen receptor（ERα and ERβ）, the proportion of the two between the imbalance, endometrial hyperplasia, and ultimately lead to endometrial lesions of endometrial cancer. Clinical observation found that genistein is represented by a plant estrogen can promote the development of endometrial and inhibit the excessive proliferation of endometrial, with anti endometrial lesion effect, but on genistein reversed endometrial high estrogen response molecular mechanisms have not been reported.. This topic is divided into three parts to study:①Effect of genistein on the expression of primary endometrial cells, Ishikawa cells and HEC-1B cells proliferation and ER and PR;②Detection of genistein report gene technology on estrogen receptor in endometrial cells - competition regulation。③The anti tumor effect of genistein on endometrial carcinoma transplanted in nude mice。.Through the above research, to observe the control effect of genistein on endometrial lesions, provide new ideas for the prevention and treatment of endometrial lesions, for further promotion of plant estrogen use in prevention and treatment of endometrial lesions and provide the basis。

子宫内膜病变发病率逐年上升，发病年龄日趋年轻化，严重影响女性身心健康。子宫内膜雌激素受体ERα、ERβ之间比例失调，内膜过度增生，最终导致子宫内膜病变甚者子宫内膜癌。临床观察发现以金雀黄素为代表的植物雌激素既可促进子宫内膜发育，又可抑制子宫内膜的过度增殖，具有抗子宫内膜病变效果，但关于金雀黄素逆转子宫内膜高雌激素应答状态分子机制尚未见研究报道。. 本课题拟分三部分进行研究：①金雀黄素对子宫内膜原代细胞、ishikawa和HEC-1B细胞增殖及细胞ER和PR表达的影响；②报告基因技术检测金雀黄素对子宫内膜细胞雌激素受体双向-竞争调节作用；③金雀黄素对子宫内膜癌荷瘤鼠的抗肿瘤效应研究。通过以上研究，观察金雀黄素对子宫内膜病变的防治效果，为子宫内膜病变的防治提供新的思路，也为进一步推广植物雌激素运用于子宫内膜病变的防治提供依据。

子宫内膜病变发病率逐年上升，发病年龄日趋年轻化，严重影响女性身心健康。子宫 内膜雌激素受体ERα、ERβ之间比例失调，内膜过度增生，最终导致子宫内膜病变甚者子 宫内膜癌。临床观察发现以金雀黄素为代表的植物雌激素既可促进子宫内膜发育，又可抑 制子宫内膜的过度增殖，具有抗子宫内膜病变效果，但关于金雀黄素逆转子宫内膜高雌激素应答状态分子机制尚未见研究报道。 .本项目主要开展植物雌激素对子宫内膜病变防治机制研究，结合前期对于子宫内膜癌细胞实验的基础，本项目模拟高低雌激素对正常子宫内膜细胞（HEEC细胞）和子宫内膜癌细胞（Ishikawa细胞）的生长状态和对细胞ERα、ERβ和 ERRα表达的影响，进一步对比观察金雀黄素和雌激素受体拮抗剂-氟维司琼对HEEC和Ishikawa细胞ERα/ERβ、ERα/ ERRα表达比例的影响,采用报告基因检测技术证实植物雌激素对子宫内膜雌激素受体的竞争表达机制，本课题重点为探讨金雀黄素逆转子宫内膜过度增殖机制的研究，发现以金雀黄素为代表的植物雌激素可通过调整子宫内膜细胞ERα/ERβ、ERα/ ERRα表达比例来抑制细胞增殖，为今后开展植物雌激素逆转子宫内膜过度增殖状态提供科学理论的佐证，拟为中医药防治子宫内膜恶性病变防治机制研究方面做出尝试。