恶性淋巴瘤中bcl-6基因异常及其临床意义的研究

Genetic alteration is important hint for precise diagnosis and prognosis of biological behavior of malignant lymphoma. Bcl-6 is cloned from lymphoma and may have distinct expression pattern and be of important genetic alteration in lymphoma. The current subject aimed to study the expression pattern of reactive hyperplasia, different types of B cell and T cell lymphoma using immunohistochemistry. By using primers encompassing frequent region of 5' nonconding region of bcl-6 gene the microdissected tissue of different types of lymphoma were investigated for mutation, combined with FISH for rearrangement frequency. Bcl-6 expressed in nuclear in germinal center of lymphonode, majority of follicular lymphoma and DLBCL. Compared with expression of bcl-2 and CD10, bcl-6 could be another important marker of centroblast derived lymphomas. The mutation frequency of Chinese DLBCL was 18.4%, lower than that in the population of other countries. Bcl-6 might partly play a role in the lymphogenesis. FISH analysis is going on and the conclusion will be confirmed on more cases. The study will provide powerful evidence on molecular level for lymphoma classification and the mechanism of lymphoma development.

本课题拟通过FISH,Southern Blot,PCR-SSCP, DNA测序等技术,系统研究淋巴瘤中bcl-6基因等异常(包括bcl-6基因的重排,5"非编码区的突变) 及与该基因的转录,蛋白表达的关系,探謆cl-6基因异常与淋巴瘤发生机制,预后的关系;用基因转染等技术在体外观察bcl-6的表达粤馨土锵赴ぬ匦院鸵┪锩舾行缘挠跋欤佣馨土鲈诜肿铀椒中秃驮ず蠹嗫靥峁┮谰荨