冬虫夏草对自然衰老大鼠卵巢功能重建与维持的分子机制：聚焦维生素D轴

The ovary is the reproductive gland of female.In the regulation of central nervous system and the hypothalamic pituitary and ovarian autocrine, paracrine, complete ovulation and endocrine function. female In middle age,the number of ovarian follicles gradually decrease and ovarian function gradually decline, reduce the secretion of estrogen. Gonadal function is an important material basis of Chinese medicine kidney deficiency. Based on previous literature and experimental research of traditional Chinese medicine, We put forward a strong relevance between "kidney essence, the main reproductive" and vitamin D axis. Scientific Hypothesis that Bushen Yijing medicine Cordyceps sinensis can regulating sex hormone secretion and related synthase gene expression. In this animal experimental study, intervention of natural aging rats with Cordyceps sinensis. Testing vitamin D axis and sex hormone secretion. And use the RNA interference (siRNA) silence ovarian granulosa cells of vitamin D receptor were observed before and after use Cordyceps sinensis. Detection estrogen producing enzymes gene expression and estrogen level. Reveal the natural aging vitamin D deficiency and deficiency of kidney essence attribute connection, molecular mechanism of Bushen Yijing drugs in the maintenance and reconstruction ovarian function.

卵巢是女性的生殖腺，在中枢神经系统-下丘脑-垂体及卵巢自分泌、旁分泌的复杂调控下，完成排卵和内分泌的功能。中年以后卵巢中卵泡数量逐渐减少，卵巢功能逐步衰退，雌激素分泌减少。性激素内环境的改变、性腺功能的下降是肾虚发生、发展的重要物质基础。基于前期文献及实验研究，提出中医"肾藏精，主生殖"功能与维生素D轴功能具有较强的关联性。补肾益精药冬虫夏草作用于卵巢维生素D轴调节性激素分泌及相关合成酶基因表达的科学假说。本课题拟从动物实验探讨冬虫夏草干预自然衰老大鼠前后维生素D轴与性激素分泌的变化。并运用RNA干扰技术（siRNA）沉默卵巢颗粒细胞维生素D受体，观察冬虫夏草干预前后雌激素生成相关酶基因表达和雌激素水平。揭示自然衰老维生素D缺乏与肾精不足证属性之间的联系，明确补肾益精药物对卵巢功能维持与重建作用的分子机制。

现代中医肾本质研究揭示肾虚证表现出性激素分泌紊乱。性腺减退是肾虚证的共同病理基础之一。但现代研究没有从整体观念探讨性激素生成在肾虚中的功能。基于文献及前期研究表明维生素D与性激素生成密切相关，维生素D缺乏与肾精亏虚之间病理生理联系密切相关，由此提出：维生素D是"肾藏精"的物质基础，补肾药冬虫夏草作用于维生素D轴调节性腺分泌及相关合成酶基因表达的科学假说。本课题拟从动物实验探讨冬虫夏草干预肾虚大鼠前后维生素D轴与性激素分泌的变化。并运用RNA干扰技术（siRNA）敲除卵巢颗粒细胞维生素D受体，观察冬虫夏草干预前后性激素生成相关酶基因表达和激素水平。揭示维生素D缺乏与肾精亏虚证候属性之间的联系，明确"肾不藏精" 客观物质基础和调控规律及冬虫夏草作用的分子机制。