HBV preC/C and preS/S gene were amplified by full-length or long-fragment polymerase chain reaction (PCR) from serum of HBsAg positive pregnant women and their newborns or liver of aborted fetus. These DNA fragments were cloned and sequenced. The results showed all nucleotide sequences of HBV strains contribute to HBV C genome type and adr type. HBV preC/C regions were conservative extremely and the heterogeneity preS/S gene was much higher. HBV nucleotide sequences of neonates and their mothers were identical; the heterogeneity of HBV nucleotide sequences in neonates was lower than that of their mother. The HBV dominant strains and weak strains in mothers can infect fetus and neonates alone or together. The heterogeneity of HBV preS/S gene in HBsAg positive pregnant women whose fetus infected HBV was much higher than that whose neonates didn't infect HBV (p<0.05). This study can offer scientific basis for further study of the relationship between HBV genomic structure and intrauterine transmission.
内容包括盐肤木叶片的有机营养物质浓度对虫瘿形成与生长的营养疚;叶组织的矿质元素含量对倍蚜瘿结倍效果的影响;在土壤-植物-昆虫生态系统内寄主叶片的养分供应、吸收和循环规律与五倍子生长的关系。成果将填补寄主植物营养论断的空白,为寄主林培育提供理论依据,在植物生理学、昆虫生态学和造林学的发展积累基础材料方面具有生要意义。
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数据更新时间:2023-05-31
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