果蝇蘑菇体神经元的单细胞转录组分析

Mushroom body, the most important structure differently located at the medullary layer of insect forebrain, is composed of special differentiated Kenyon cells. Kenyon cells are further classified into three subtypes, including α/β, α’/β’ and γ subtypes that control the α/β, α’/β’ and γ lobe, respectively. The classification of Kenyon cell subtypes is not just up to their different structures, it also reflects differences in development and functions. To further understand the functional differences and related mechanisms in these subtypes of Kenyon cells, we will use different subtype-specific Gal4 by driving UAS-GFP to label α/β, α’/β’ and γ neurons, and then choose single cell from marked GFP for high-throughput sequencing and analysis of transcriptome. Through analysising the differences of transcriptomes from different subtypes and different developmental stages of Kenyon cells, we can further understand molecular mechanisms of structures and functions of Kenyon cells in Drosophila, which will provide new ideas and insights in the functions of mushroom body neurons in learning and memory as well as other related functions.

蘑菇体（Mushroom Body）是昆虫大脑行使功能的重要结构，位于昆虫前脑叶髓层，由特殊分化的Kenyon细胞组成。Kenyon细胞又分为α/β、α’/β’和γ三种亚型，分别支配蘑菇体的α/β、α’/β’和γ叶。Kenyon细胞亚型的分类不只是结构上的不同，还反映了在发育和功能上的区别。为了进一步解析蘑菇体各类型Kenyon细胞功能差异及其分子机制，我们利用组织细胞特异性的Gal4，启动UAS-GFP表达，分别标记α/β、α’/β’和γ三种不同类型的蘑菇体Kenyon细胞，并挑选单细胞进行cDNA文库构建和高通量测序与分析。通过比较不同类型以及同一类型在不同发育时期的蘑菇体Kenyon细胞的转录组差异，解析果蝇大脑蘑菇体各结构组分和功能的分子基础，为揭示蘑菇体神经元在学习记忆以及其他相关行为中的功能提供新思路和新见解。

蘑菇体（Mushroom Body）是昆虫大脑行使功能的重要结构，位于昆虫前脑叶髓层，由特殊分化的Kenyon细胞组成。Kenyon细胞又分为α/β、α’/β’和γ三种亚型，分别支配蘑菇体的α/β、α’/β’和γ叶。Kenyon细胞亚型的分类不只是结构上的不同，还反映了在发育和功能上的区别。为了进一步解析蘑菇体各类型Kenyon细胞功能差异及其分子机制，我们利用交叉标记法（intersectional system）分别特异性的标记α/β、α’/β’和γ三种不同类型的蘑菇体Kenyon细胞，并手工挑取不同类型的细胞进行cDNA文库构建和高通量测序与分析。通过比较不同类型蘑菇体Kenyon细胞的转录组差异，解析果蝇大脑蘑菇体各结构组分和功能的分子基础，为揭示蘑菇体神经元在学习记忆以及其他相关行为中的功能提供新思路和新见解。