茶树叶片细胞壁与氟的结合机制研究

Tea plant(Camellia sinensis (L.) O.Kuntze) has very strong capacity to absorb fluorine from soil and the fluorine is mainly located in mature leaf. However, the mechanism in tea plant uptake fluorine hasn't been explained and the conformation of fluorine in cell has remained unclear. The earlier investigation in the distribution of fluorine in cell showed that the content of fluorine in the cell wall is more than 50% of the total content, which indicates the cell wall is the main sub-cell where fluorine is located. In the present research, some new methods of fluorine chemistry, carbohydrate chemistry, physics and biology will be used to study the combination of fluorine with compounds in the cell wall. The relation between the structure of cell wall and fluorine will be analyzed and the feature of cell wall fluorine will also be studied in the present project. The aim of this study is to understand the cumulative properties of fluorine in cell wall of tea fresh leaf. Finally, we hope to provide theoretical basis to the research on fluorine in situ in tea leaf, mechanism in fluorine absorption by tea plant and reducing fluorine content in old leaf.

茶树是自然界高度富氟的植物，并主要富集在成熟叶片中，而茶叶氟的富集机理及存在形态尚不明了，极大地影响到对低档茶叶的安全利用。本课题前期通过分析细胞中氟的分布，发现茶树叶片细胞壁氟占总氟含量的50%以上，揭示出细胞壁是氟存在的主要亚细胞。基于此，该课题利用氟化学、糖化学、物理学以及生物学方法进一步研究细胞壁组成物质与氟的结合方式，分析细胞壁结构与氟结合的关系，探究细胞壁氟的特性，以探明氟在细胞壁的累积特性，一方面为茶树叶片中氟的原位形态研究提供基础，另一方面为茶树富集氟的机制以及茶叶降氟研究提供重要的理论依据。