水体悬浮颗粒物上藻-菌生物膜联合降解PAHs的作用研究

Suspended Particulate Matters (SPM) is the crucial carrier of organic contaminants such as Polycyclic Aromatic Hydrocarbons(PAHs). In addition ,the surface of SPM also provides a vital environmental condition for the microalgae and bacteria to form polymicrobial aggregates such as biofilms. With high bioactivity, biofilms are proved to play an important role to the fate of organic pollutants. Therefore, the key point of this research is based on the common biodegradation of PAHs with organisms (bacterias and microalgaes with different ecological niches) in the biofim that absorbed on the SPM surface. Firstly, the spatial distribution of PAHs and their metabolites in SPM of the pearl river estuary will be investigated after the development of determination methods, and than make a in-depth insigh about the degradation mechanisms of PAHs by the biofilm of algae-germ cobination based on the metabolites production, characteristics of degradation genes and the effects of extracellular polymeric substances (EPS) of biofilm. So, this study can help us for a better understanding of the fate of PAHs by the effects of bioprocess within the multi-interfaces of SPM and biofilm, and also the control and environmental risks of organic pollutants in estuary regions.

水体悬浮颗粒物(SPM)界面是多环芳烃(PAHs)等有机污染物的重要载体，同时也为细菌、微藻等组成的高生物活性的生物膜提供重要的生长条件，由SPM介导的生物膜中的生物代谢作用对污染物的环境行为和生态效应也具有重要作用。因此本项目将以PAHs污染胁迫下SPM界面上不同生态位的细菌和微藻的生物降解过程作为主要研究内容，结合珠江河口的野外调查以及实验室模拟，研究PAHs及其代谢产物在实际水体SPM中的时空分布规律，并且从降解产物、降解基因共性等以及生物膜中胞外聚合物等角度深入探讨藻-菌联合降解对水体SPM中PAHs的生物降解过程。同时探讨藻-菌生物膜对PAHs污染胁迫的响应机制，并且研究其中EPS成分对PAHs生物利用的影响因素。旨在阐明水环境中SPM、生物膜等多界面中PAHs的生物降解过程以及转化规律，为河口及近岸海域的PAHs污染控制以及生态环境评价提供可靠的理论支持和实践基础。

水体悬浮颗粒物(SPM)界面是多环芳烃(PAHs)等有机污染物的重要载体，同时也为细菌、微藻等组成的高生物活性的生物膜提供重要的生长条件，由SPM介导的生物代谢作用对污染物的环境行为和生态效应也具有重要作用。本项目以PAHs污染胁迫下的细菌和微藻的生物降解过程作为主要研究内容，结合珠江河口的野外调查以及实验室模拟，探讨了PAHs及其代谢产物在实际水体SPM中的时空分布规律，并且从降解产物、降解基因共性等以及生物膜中胞外聚合物等角度阐明了藻-菌联合降解对水体SPM中PAHs的生物降解过程。同时，我们研究了藻-菌联合体系PAHs污染胁迫的响应机制，阐明了两者对污染物的协同降解机制。项目的开展为河口及近岸海域的PAHs污染控制以及生态环境评价提供可靠的理论支持和实践基础。目前已发表标注过的SCI论文15篇，其中通讯作者论文14篇，包括《Environmental Science & Technology》、《Analytical Chemistry》等科学顶级期刊。