基于软骨细胞骨架蛋白探讨温针灸对骨关节炎的调节作用

In recent years, osteoarthritis (OA) has become the advantage diseases with acupuncture therapy, as chondrocytes in cartilage cells only, its function and morphology in the pathogenesis of OA are highly concerned. This subject is on the basis research of acupuncture treatment on joint diseases and cytoskeleton function, with papain preparation of OA in the rat model, and through different methods after acupuncture intervention, in order to research the regulation of inflammatory response of acupuncture and moxibustion on OA model rats. Targeting on cytoskeletal proteins in cartilage to study the regulation of endogenous cartilage with warm acupuncture, and focusing on RhoA / LIMK1 / Cofilin signaling pathway as a target, also using pathway inhibitor, we in tend to explore acupuncture effect on the signaling pathway. We aim to clarify the regulation of inflammatory reaction and molecular mechanisms of warm acupuncture on OA rat model through the above research, and further deepen the mechanism of warm acupuncture treatment on bone and joint diseases in the respect of inflammation mechanisms, so as to provide new experimental evidence for clinical.

近年来，骨关节炎(OA)已成为针刺治疗的优势病种，软骨细胞作为软骨中唯一的细胞，其功能和形态结构在OA发病机制中的意义受到高度关注。本课题在申请者对针灸治疗骨关节疾病研究以及细胞骨架功能研究的基础上，以木瓜蛋白酶制备OA大鼠模型，不同针灸方法干预，研究针灸对骨关节炎模型大鼠炎症反应的调节作用；以软骨细胞骨架蛋白为切入点，观察温针灸对内源性软骨细胞骨架蛋白的调节作用，并以RhoA/LIMKl/Cofilin信号通路为靶点，结合通路抑制剂，探索温针灸对该通路的调节作用。通过上述研究，以期阐明温针灸对OA模型大鼠骨关节炎症反应的调节作用及分子机制，进一步深化温针灸治疗骨关节疾病的炎症机制机理，为临床提供新的实验依据。

近年来，骨关节炎(OA)已成为针刺治疗的优势病种，软骨细胞作为软骨中唯一的细胞，其功能和形态结构在OA发病机制中的意义受到高度关注。本课题在申请者对针灸治疗骨关节疾病研究以及细胞骨架功能研究的基础上，以木瓜蛋白酶制备OA大鼠模型，不同针灸方法干预，研究针灸对骨关节炎模型大鼠炎症反应的调节作用；以软骨细胞骨架蛋白为切入点，观察温针灸对内源性软骨细胞骨架蛋白的调节作用，并以RhoA/LIMKl/Cofilin信号通路为靶点，结合通路抑制剂，探索温针灸对该通路的调节作用。通过上述研究，以期阐明温针灸对OA模型大鼠骨关节炎症反应的调节作用及分子机制，进一步深化温针灸治疗骨关节疾病的炎症机制机理，为临床提供新的实验依据。