生物分子功能化碳纳米材料复合物修饰电极电化学传感分析的研究

At present, it is an important research area in life analytical chemistry that preparation of biomolecule-functionalized carbon nanomaterial composite modified electrodes which possess biocompatibility and special recognition function. This program will study on the synthesis of biomolecules-functionalized carbon nanomaterial and the preparation as well as application of its composite modified electrode. From the beginning, the carbon nanomaterials are biomolecules-functionalized, and then formed carbon nanomaterial composites combined ionic liquid or magnetic material which possesses biocompatibility. Furthermore, they are assembled on the surface of the electrodes, and construct bio-interface which has biologic special recognition and biologic catalytic function for the substrates. We hope to develop new methods and techniques in biosensors with high sensitivity and high selectivity. Meanwhile, we will discuss the interaction of biomolecules, nanomaterials and ionic liquids, and investigate mechanism of electrocatalysis of modified electrodes, providing theory and experimental base for setting up new methods as well as obtaining information in biological system.

具备生物相容性和特殊识别功能的生物分子功能化碳纳米材料复合物修饰电极的制备是目前生命分析化学的前沿领域，本项目拟开展生物分子功能化纳米材料的合成及其修饰电极的制备和应用研究。从碳纳米材料表面生物分子功能化出发，引入离子液体和磁性材料，制备具有优良生物相容性的碳纳米材料复合物。进一步组装到电极表面，构建对底物具有生物特异性识别和生物催化功能的生物界面，建立生物分子识别、催化和传感的技术，发展高灵敏度和高选择性的生物传感新方法和新技术。同时探讨生物分子、纳米材料和离子液体相互作用，研究修饰电极的电催化机理等，为建立生物体系中信息的获取和测试方法提供理论和实验基础。

本项目为科学部主任基金，研究期限为一年。 在这一年中我们主要开展了功能碳纳米材料的制备及其在生物传感界面上的应用研究.完成了离子液体功能化碳纳米复合材料的制备，构建了具有优良生物相容性的生物分子功能化的传感界面，发展了对过氧化氢、胆固醇、多目标DNA等生物分子具有高灵敏度和高选择性的检测新方法。同时探讨了生物分子、纳米材料和离子液体相互作用，研究了所构建的修饰电极的电催化机理。本项目的开展有效地拓展了功能化碳纳米材料在生物传感界面的应用。