相变型多功能分子探针显像及治疗肝癌射频消融后残余灶

Radiofrequency ablation (RFA) for liver cancer has several problems such as residual tumor that would cause local recurrence and metastasis. Early detection and treatment of tumor remnants helps to improve the curative effect of RFA, but now it is difficult to use a single imaging technology to detect the residual tumor in the early stage as each traditional medical imaging technology has its own defects. In this project, we will develop a new type of multi-functional molecular probe (folate receptor targeted, paclitaxel and Fe3O4 loaded perfluorocarbon nanoparticles) that can be targeted to hepatoma cells and can generate liquid - gas phase transition with the application of low intensity focus ultrasound, so as to be used for ultrasound, MRI and CT molecular imaging and therapy of the residual tumor. The targeted imaging and therapy effect will be studied in vitro and in vivo. We aim to resolve the problem in early diagnosis and treatment of the residual liver cancer after RFA, and provide a new method for effective minimally invasive treatment of liver cancer.

射频消融（RFA）治疗肝癌存在肿瘤残留等问题，易造成肿瘤局部复发与转移。早期发现与治疗肿瘤残余灶有助于提高RFA疗效，但是各种传统医学影像技术均存在各自的缺陷，目前难以利用单一的影像技术做到早期发现肿瘤残余灶，是限制进一步提高RFA疗效的关键因素之一。针对上述问题，本项目拟在前期工作基础上，研制一种具有液-气相变性能的新型多功能超声分子探针——叶酸靶向载药氟碳纳米粒，通过建立肝癌射频消融后残癌模型，使其主动识别肝癌细胞并浓聚于残癌部位，并通过一定能量的超声辐照，使纳米粒发生液气相变形成微泡，以超声分子成像为核心，结合MRI、CT等其它成像技术，针对射频消融后的残余灶进行多模式、多参数的分子成像，同时在分子成像的监控下释放药物靶向杀灭残癌组织，研究其对残余灶的多模态分子显像及靶向治疗效果，以期解决肝癌RFA治疗中存在的残余肿瘤早期诊断与治疗难题，为高效的肝癌微创治疗提供一种新的方法与思路。

热消融治疗实体肿瘤存在肿瘤残留等问题，易造成肿瘤局部复发与转移。本项目成功研制一种具有液-气相变性能的新型多功能超声分子探针——叶酸修饰的载药相变液态氟碳微球，并证明了其能较好地靶向于肿瘤内，从而在治疗超声作用后发生相变产生超声微泡，最终实现同时增强超声显影及消融效果的目的，为高效的肿瘤微创治疗提供一种新的方法与思路。