基于不同信号通路探讨督脉电针对脊髓损伤后神经再生的作用机制

To explore the mechanism of signal pathway after spinal cord injury with electroacupuncture treatments through the modern molecular biology technology. Using fMRI to observe changes of the brain function area,and using the immune eohistochemistry, RT-PCR ,western blotting method to analyse on gene and protein expressions of wnt,notch signal pathway with combination of transmission electron microscopy observation to see ultrastructure changes of neuron and axon, reveal on material foundation and inner link between nerve regeneration , brain plasticity ,functional restoration of axonal conduction and differences in protein material basis. To further explore the mechanism on synergistic effect of wnt, notch signal pathway to regulate directional differentiation of neural stem cells into neurons with acupuncture treatment for spinal cord injury,to explore the mechanism of development and growth of neurons and neurite, to give a reasonable explansion from different aspects of acupuncture treatment on SCI, to see the application through gene therapy of treating the neuron system diseases, expanding the acupuncture treatment range, and it has very important scientific significance to promote the modernization and internationalization of traditional Chinese medicine.

本课题应用现代分子生物学技术，从整体水平观察了大脑功能区的变化情况，从组织分子水平观测了Wnt、Notch信号通路中的基因和蛋白表达情况，结合透射电子显微镜观察受损脊髓组织的神经元、轴突的变化，揭示电针干预大鼠脊髓损伤后神经再生修复、脑功能重塑的效应物质变化规律；进一步探讨Wnt、Notch信号的协同作用对脊髓损伤后神经干细胞向神经元的定向分化的调控机制，为探索针灸临床治疗脊髓损伤提供科学的实验证据。本研究不仅仅是探讨针灸治疗脊髓损伤的作用机理，还是对治疗脊髓损伤和促进神经再生功能新疗法的开发，拓展了人们在基因治疗方面的应用，扩大了针灸的治疗范围，对促进中医药的现代化和国际化都具有十分重要的科学意义。

本课题应用现代分子生物学技术，从整体水平观察了脊髓损伤局部和大脑功能区的变化情况，从组织分子水平观测了Wnt、Notch、ERK、JNK、Akt/mTOR/PTEN信号通路中的基因和蛋白表达情况，揭示电针干预大鼠脊髓损伤后神经干细胞向神经元的定向分化、神经再生修复、轴突再生、脑功能重塑的效应物质变化规律；采用核磁共振（f-MRI）扫描了脊髓损伤后大鼠功能区及受损脊髓组织形态学改变，揭示了脊髓神经再生、重建、脑功能重塑和信号通路的物质基础及其内在联系，阐述‘通督启神法’”的中医学理论，揭示“督脉-脊髓-脑”的科学内涵，为探索针灸临床治疗脊髓损伤提供科学的实验证据。